Flow Cytometry Protocols

  • Teresa S. Hawley
  • Robert G. Hawley

Part of the Methods in Molecular Biology book series (MIMB, volume 1678)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Howard M. Shapiro
    Pages 1-10
  3. Dongxia Lin, Holden T. Maecker
    Pages 37-47
  4. Kah Teong Soh, Paul K. Wallace
    Pages 49-77
  5. John P. Nolan, Erika Duggan
    Pages 79-92
  6. Lili Wang, Adolfas K. Gaigalas, James Wood
    Pages 93-110
  7. Joshua Paterson, Laurie E. Ailles
    Pages 111-138
  8. Katherine M. McKinnon
    Pages 139-150
  9. Patricia Lovelace, Holden T. Maecker
    Pages 151-166
  10. James W. Jacobberger, R. Michael Sramkoski, Tammy Stefan, Philip G. Woost
    Pages 203-247
  11. Joseph D. Tario Jr., Alexis N. Conway, Katharine A. Muirhead, Paul K. Wallace
    Pages 249-299
  12. Cornelis J. H. Pronk, David Bryder
    Pages 301-319
  13. Maurice. R. G. O’Gorman
    Pages 321-345
  14. Maik Herbig, Martin Kräter, Katarzyna Plak, Paul Müller, Jochen Guck, Oliver Otto
    Pages 347-369
  15. László Ujlaky-Nagy, Péter Nagy, János Szöllősi, György Vereb
    Pages 393-419
  16. Jessica P. Houston, Zhihua Yang, Jesse Sambrano, Wenyan Li, Kapil Nichani, Giacomo Vacca
    Pages 421-446
  17. William G. Telford
    Pages 447-479
  18. Howard M. Shapiro
    Pages 481-488
  19. Back Matter
    Pages 489-492

About this book


This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellular cytokine staining, apoptosis analysis, cell cycle analysis, tracking cell proliferation, and monitoring protein-protein interactions using FRET. Some developing methodologies that explored are mass cytometry, vesicle flow cytometry, time-resolved flow cytometry, and real-time label-free deformability cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Flow Cytometry Protocols, Fourth Edition is a valuable resource for researchers and scientists who are interested in continuing and/or expanding their knowledge of flow cytometry.


intracellular protein biomarkers cytokine staining apoptosis analysis cell cycle FRET antibodies proteins

Editors and affiliations

  • Teresa S. Hawley
    • 1
  • Robert G. Hawley
    • 2
  1. 1.Flow Cytometry Section, Research Technologies BranchNational Institute of Allergy and Infectious Diseases, National Institutes of HealthBethesdaUSA
  2. 2.Department of Anatomy and Regenerative Biology, School of Medicine and Health SciencesGeorge Washington UniversityWashingtonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7344-6
  • Online ISBN 978-1-4939-7346-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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