Methods and Protocols

  • L. Mark Lagrimini

Part of the Methods in Molecular Biology book series (MIMB, volume 1676)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Cell, Tissue, and Organ Culture and Maize Transformation

    1. Front Matter
      Pages 1-1
    2. Jennifer A. Raji, Bronwyn Frame, Daniel Little, Tri Joko Santoso, Kan Wang
      Pages 15-40
    3. Heng Zhong, Sivamani Elumalai, Samson Nalapalli, Lee Richbourg, Anna Prairie, David Bradley et al.
      Pages 41-59
  3. Gene Silencing and Generation of Mutant Populations

    1. Front Matter
      Pages 95-95
    2. Shangang Jia, Aixia Li, Chi Zhang, David Holding
      Pages 97-108
    3. Camilla Betti, Joost Schymkowitz, Frederic Rousseau, Eugenia Russinova
      Pages 109-127
    4. Tao Zhou, Xuedong Liu, Zaifeng Fan
      Pages 141-150
  4. Plant Gene Expression

    1. Front Matter
      Pages 151-151
    2. Prakitchai Chotewutmontri, Nicholas Stiffler, Kenneth P. Watkins, Alice Barkan
      Pages 165-183
    3. Qing Li, Peter J. Hermanson, Nathan M. Springer
      Pages 185-196
    4. Levi G. Lowder, Aimee Malzahn, Yiping Qi
      Pages 197-214
  5. Plant Metabolic Networks

    1. Front Matter
      Pages 215-215
    2. Maria Emilia Dueñas, Adam D. Feenstra, Andrew R. Korte, Paige Hinners, Young Jin Lee
      Pages 217-231
  6. Back Matter
    Pages 233-234

About this book


This volume discusses techniques used for the molecular characterization of maize. This book is divided into 4 parts: cell, tissue, and organ culture and maize transformation; gene silencing and generation of mutant populations; plant gene expression; and plant metabolic networks. The chapters cover a range of topics, such as growing and propagating maize in the laboratory, greenhouse and field studies, screening mutagenic population, characterizing the genome, describing protein and metabolic regulatory networks, and generating transgenic plants for gene knock-out and over expression purposes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Maize: Methods and Protocols is a valuable resource for everyone who is interested in maize research.


Corn yield Plant epigenetics Maize proteome Plant metabolic networks Mutagenic populations

Editors and affiliations

  • L. Mark Lagrimini
    • 1
  1. 1.Department of Agronomy and HorticultureUniversity of Nebraska – LincolnLincolnUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7314-9
  • Online ISBN 978-1-4939-7315-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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