Plant Germline Development

Methods and Protocols

  • Anja Schmidt

Part of the Methods in Molecular Biology book series (MIMB, volume 1669)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Plant Germline Development: From Investigation to Application

    1. Front Matter
      Pages 1-1
    2. Hannes Vogler, Ueli Grossniklaus, Afif Hedhly
      Pages 3-15
    3. Joann A. Conner, Peggy Ozias-Akins
      Pages 17-34
  3. Clearing, Imaging Techniques, and Immunolocalization to Study Cell Lineage, Cell Type Specificity, Cytoskeleton, Cell Cycle, Signaling, and Hormones

    1. Front Matter
      Pages 35-35
    2. Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, Tomokazu Kawashima
      Pages 47-54
    3. Burcu Nur Keçeli, Nico De Storme, Danny Geelen
      Pages 77-85
    4. Antony M. Chettoor, Matthew M. S. Evans
      Pages 95-101
    5. Mário Luís da Costa, Ana Lúcia Lopes, Maria Isabel Amorim, Sílvia Coimbra
      Pages 117-137
  4. Chromosome Spreading, Whole Mount In Situ, and Transmission Electron Microscopy (TEM)

    1. Front Matter
      Pages 139-139
    2. Andrea Bleckmann, Thomas Dresselhaus
      Pages 159-171
  5. Isolation of Specific Cells of the Male and Female Germlines

    1. Front Matter
      Pages 191-191
    2. Scott D. Russell, Daniel S. Jones, Sarah Anderson, Xinkun Wang, Venkatesan Sundaresan, Xiaoping Gou
      Pages 211-219
  6. Omics Technologies and Bioinformatic Analyses

    1. Front Matter
      Pages 235-235
    2. Takashi Okamoto
      Pages 251-263
    3. Benjamin Peters, Jack Aidley, Murray Cadzow, David Twell, Lynette Brownfield
      Pages 275-293
    4. Weihong Qi, Ralph Schlapbach, Hubert Rehrauer
      Pages 295-307
  7. Technologies with Outreach for Plant Manipulation, Studies of Pathways, and Applications in Crop Species

    1. Front Matter
      Pages 337-337
    2. Iris Heidmann, Marco Di Berardino
      Pages 339-354
    3. Emily Wu, Zuo-Yu Zhao
      Pages 355-364
    4. Shai J. Lawit, Mark A. Chamberlin
      Pages 377-386
    5. Pavel Reichman, Nico Dissmeyer
      Pages 387-406
  8. Back Matter
    Pages 417-422

About this book


This detailed volume explores common and numerous specialized methods to study various aspects of plant germline development and targeted manipulation, including imaging and hybridization techniques to study cell-type specification, cell lineage, signaling and hormones, cell cycle, and the cytoskeleton. In addition, cell-type specific methods for targeted ablation or isolation are provided, protocols to apply “omics” technologies and to perform bioinformatics data analysis, as well as methods relevant for aspects of biotechnology or plant breeding. This includes protocols that are relevant for the targeted manipulation of pathways, for crop plant transformation, or for conditional induction of phenotypes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Germline Development: Methods and Protocols serves as a comprehensive guide not only to studying basic questions related to different aspects of plant reproductive development but also for state of the art methods, in addition to being a source of inspiration for new approaches and research questions in many laboratories.


Plant reproduction Cell lineage Imaging techniques Crop plant transformation Developmental genetics Crop improvement

Editors and affiliations

  • Anja Schmidt
    • 1
  1. 1.Centre for Organismal StudiesUniversity of HeidelbergHeidelbergGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7285-2
  • Online ISBN 978-1-4939-7286-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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