Difference Gel Electrophoresis

Methods and Protocols

  • Kay Ohlendieck

Part of the Methods in Molecular Biology book series (MIMB, volume 1664)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. Difference Gel Electrophoresis

    1. Front Matter
      Pages 15-15
    2. Kay Ohlendieck
      Pages 17-24
    3. Elisa Robotti, Emilio Marengo
      Pages 25-39
    4. Abduladim Hmmier, Paul Dowling
      Pages 41-50
  4. DIGE Applications

    1. Front Matter
      Pages 51-51
    2. Diksha Dani, Norbert A. Dencher
      Pages 53-68
    3. Doreen Ackermann, Simone König
      Pages 69-78
    4. Taras Stasyk, Lukas Alfons Huber
      Pages 79-86
    5. Sandra Murphy, Paul Dowling
      Pages 109-114
  5. Comparative DIGE Tissue Proteomics

    1. Front Matter
      Pages 115-115
    2. Cecilia Gelfi, Daniele Capitanio
      Pages 117-136
    3. Alessio Di Luca, Ruth Hamill, Anne Maria Mullen, Giuliano Elia
      Pages 137-152
    4. Shotaro Kamata, Isao Ishii
      Pages 153-166
    5. Jarlath E. Nally, Simone Schuller
      Pages 167-183
    6. Joanna Nynca, Mariola A. Dietrich, Andrzej Ciereszko
      Pages 203-219
  6. Auxillary DIGE Methods

    1. Front Matter
      Pages 221-221
    2. Sandra Murphy, Kay Ohlendieck
      Pages 223-232
    3. Paul Dowling, Kay Ohlendieck
      Pages 245-257
  7. DIGE Verification Analysis

    1. Front Matter
      Pages 259-259
    2. Martin Landsberger, Heinrich Brinkmeier
      Pages 287-299
    3. Rustam R. Mundegar, Margit Zweyer, Dieter Swandulla
      Pages 301-309
  8. Back Matter
    Pages 311-315

About this book


This volume provides a comprehensive study of this key method of gel-based proteomics. Chapters inDifference Gel Electrophoresis: Methods and Protocols detail methods on principles of differential protein labeling, two-dimensional gel electrophoresis, techniques on optimized proteomic workflows, advanced mass spectrometry for protein identification, application of basic biological research, and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.


DIGE fluorescence image analysis gel-based proteomics two-dimensional gel electrophoresis protein identification mass spectrometry

Editors and affiliations

  • Kay Ohlendieck
    • 1
  1. 1.Department of BiologyMaynooth University, National University of IrelandMaynooth, Co. KildareIreland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7267-8
  • Online ISBN 978-1-4939-7268-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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