Methods and Protocols

  • Laurent Chavatte

Part of the Methods in Molecular Biology book series (MIMB, volume 1661)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Bioinformatic Tools for Selenoprotein Identification and Evolution

    1. Front Matter
      Pages 1-1
    2. Didac Santesmasses, Marco Mariotti, Roderic Guigó
      Pages 17-28
  3. Selenoprotein Biosynthesis

    1. Front Matter
      Pages 41-41
    2. Bradley A. Carlson, Byeong Jae Lee, Petra A. Tsuji, Paul R. Copeland, Ulrich Schweizer, Vadim N. Gladyshev et al.
      Pages 43-60
    3. Mark H. Pinkerton, Paul R. Copeland
      Pages 93-101
    4. Brian K. Dalley, Lisa Baird, Michael T. Howard
      Pages 103-123
    5. Anne-Sophie Gribling-Burrer, Gilbert Eriani, Christine Allmang
      Pages 125-141
  4. Selenoprotein Detection and Quantification

    1. Front Matter
      Pages 143-143
    2. Belén Callejón-Leblic, Gema Rodríguez-Moro, Tamara García-Barrera, José Luis Gómez-Ariza
      Pages 163-175
    3. Sun Hee Yim, Ryuta Tobe, Anton A. Turanov, Bradley A. Carlson
      Pages 177-192
    4. Jordan Sonet, Sandra Mounicou, Laurent Chavatte
      Pages 193-203
    5. Elisa Castañeda Santa Cruz, J. Susanne Becker, J. Sabine Becker, Alessandra Sussulini
      Pages 219-227
  5. Selenoprotein Overexpression, Purification, and Activity

    1. Front Matter
      Pages 229-229
    2. Qing Cheng, Elias S. J. Arnér
      Pages 231-240
    3. Zhengqi Zhang, Jun Liu, Sharon Rozovsky
      Pages 241-263
    4. Jun Liu, Qingqing Chen, Sharon Rozovsky
      Pages 265-283
    5. Lionel Tarrago, Emmanuel Oheix, Zalán Péterfi, Vadim N. Gladyshev
      Pages 285-299
  6. In Vivo Studies

  7. Back Matter
    Pages 337-340

About this book


This volume aims at providing an update on state of the art methodologies to study various aspects of selenoprotein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Selenoproteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.


redox enzymes biosynthesis SECIS binding proteins TrxRD

Editors and affiliations

  • Laurent Chavatte
    • 1
  1. 1.Centre International de Recherche en Infectiologie, CIRILyonFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7257-9
  • Online ISBN 978-1-4939-7258-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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