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Selenoproteins

Methods and Protocols

  • Laurent Chavatte
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 1661)

Table of contents

  1. Front Matter
    Pages i-xv
    PDF

  2. Bioinformatic Tools for Selenoprotein Identification and Evolution

    1. Front Matter
      Pages 1-1
      PDF
    2. SECISearch3 and Seblastian: In-Silico Tools to Predict SECIS Elements and Selenoproteins
      Marco Mariotti
      Pages 3-16
    3. Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins
      Didac Santesmasses, Marco Mariotti, Roderic Guigó
      Pages 17-28
    4. SelGenAmic: An Algorithm for Selenoprotein Gene Assembly
      Liang Jiang, Qiong Liu
      Pages 29-39

  3. Selenoprotein Biosynthesis

    1. Front Matter
      Pages 41-41
      PDF
    2. Selenocysteine tRNA[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification, and Sequencing
      Bradley A. Carlson, Byeong Jae Lee, Petra A. Tsuji, Paul R. Copeland, Ulrich Schweizer, Vadim N. Gladyshev et al.
      Pages 43-60
    3. Identification and Characterization of Proteins that Bind to Selenoprotein 3′ UTRs
      Eric M. Cockman, Donna M. Driscoll
      Pages 61-71
    4. Specific Chemical Approaches for Studying Mammalian Ribosomes Complexed with Ligands Involved in Selenoprotein Synthesis
      Olga Kossinova, Alexey Malygin, Alain Krol, Galina Karpova
      Pages 73-92
    5. In Vitro Translation Assays for Selenocysteine Insertion
      Mark H. Pinkerton, Paul R. Copeland
      Pages 93-101
    6. Studying Selenoprotein mRNA Translation Using RNA-Seq and Ribosome Profiling
      Brian K. Dalley, Lisa Baird, Michael T. Howard
      Pages 103-123
    7. Modification of Selenoprotein mRNAs by Cap Tri-methylation
      Anne-Sophie Gribling-Burrer, Gilbert Eriani, Christine Allmang
      Pages 125-141

  4. Selenoprotein Detection and Quantification

    1. Front Matter
      Pages 143-143
      PDF
    2. Total Selenium Quantification in Biological Samples by Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
      Véronique Vacchina, Jean Dumont
      Pages 145-152
    3. Quantification of SeMet and SeCys in Biological Fluids and Tissues by Liquid Chromatography Coupled to Inductively Coupled Plasma Mass Spectrometry (HPLC-ICP MS)
      Véronique Vacchina, Katarzyna Bierla, Joanna Szpunar, Ryszard Lobinski
      Pages 153-162
    4. Simultaneous Speciation of Selenoproteins and Selenometabolites in Plasma and Serum
      Belén Callejón-Leblic, Gema Rodríguez-Moro, Tamara García-Barrera, José Luis Gómez-Ariza
      Pages 163-175
    5. Radioactive 75Se Labeling and Detection of Selenoproteins
      Sun Hee Yim, Ryuta Tobe, Anton A. Turanov, Bradley A. Carlson
      Pages 177-192
    6. Nonradioactive Isotopic Labeling and Tracing of Selenoproteins in Cultured Cell Lines
      Jordan Sonet, Sandra Mounicou, Laurent Chavatte
      Pages 193-203
    7. Detection of Selenoproteins by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP MS) in Immobilized pH Gradient (IPG) Strips
      Jordan Sonet, Sandra Mounicou, Laurent Chavatte
      Pages 205-217
    8. Imaging of Selenium by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in 2-D Electrophoresis Gels and Biological Tissues
      Elisa Castañeda Santa Cruz, J. Susanne Becker, J. Sabine Becker, Alessandra Sussulini
      Pages 219-227

  5. Selenoprotein Overexpression, Purification, and Activity

    1. Front Matter
      Pages 229-229
      PDF
    2. Overexpression of Recombinant Selenoproteins in E. coli
      Qing Cheng, Elias S. J. Arnér
      Pages 231-240
    3. Preparation of Selenocysteine-Containing Forms of Human SELENOK and SELENOS
      Zhengqi Zhang, Jun Liu, Sharon Rozovsky
      Pages 241-263
    4. Selenocysteine-Mediated Expressed Protein Ligation of SELENOM
      Jun Liu, Qingqing Chen, Sharon Rozovsky
      Pages 265-283
    5. Monitoring of Methionine Sulfoxide Content and Methionine Sulfoxide Reductase Activity
      Lionel Tarrago, Emmanuel Oheix, Zalán Péterfi, Vadim N. Gladyshev
      Pages 285-299
    6. Selective Evaluation of Thioredoxin Reductase Enzymatic Activities
      Elias S. J. Arnér
      Pages 301-309

  6. In Vivo Studies

    1. Front Matter
      Pages 311-311
      PDF
    2. Association of Single Nucleotide Polymorphisms in Selenoprotein Genes with Cancer Risk
      Catherine Méplan
      Pages 313-324
    3. Identification of Genetic Disorders Causing Disruption of Selenoprotein Biosynthesis
      Erik Schoenmakers, Krishna Chatterjee
      Pages 325-335
  7. Back Matter
    Pages 337-340
    PDF

About this book

Introduction

This volume aims at providing an update on state of the art methodologies to study various aspects of selenoprotein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Selenoproteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Keywords

redox enzymes biosynthesis SECIS binding proteins TrxRD

Editors and affiliations

  • Laurent Chavatte
    • 1
  1. 1.Centre International de Recherche en Infectiologie, CIRILyonFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7258-6
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7257-9
  • Online ISBN 978-1-4939-7258-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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