Extracellular Vesicles

Methods and Protocols

  • Winston Patrick Kuo
  • Shidong Jia

Part of the Methods in Molecular Biology book series (MIMB, volume 1660)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Mingyi Shang, John S. Ji, Chao Song, Bao Jun Gao, Jason Gang Jin, Winston Patrick Kuo et al.
    Pages 1-14
  3. Winston Patrick Kuo, John C. Tigges, Vasilis Toxavidis, Ionita Ghiran
    Pages 15-22
  4. Isolation of Extracellular Vesicles

    1. Front Matter
      Pages 23-23
    2. Yi-Hsing Hsiao, Chihchen Chen
      Pages 43-54
    3. Ketil W. Pedersen, Bente Kierulf, Axl Neurauter
      Pages 65-87
  5. Purification of Extracellular Vesicles

    1. Front Matter
      Pages 89-89
    2. Peter N. Brown, Hang Yin
      Pages 91-103
    3. Rebecca E. Lane, Darren Korbie, Matt Trau, Michelle M. Hill
      Pages 111-130
  6. Characterization of Extracellular Vesicles

    1. Front Matter
      Pages 131-131
    2. Hyungsoon Im, Katherine Yang, Hakho Lee, Cesar M. Castro
      Pages 133-141
    3. Emma J. K. Kowal, Dmitry Ter-Ovanesyan, Aviv Regev, George M. Church
      Pages 143-152
    4. Pauline Carnell-Morris, Dionne Tannetta, Agnieszka Siupa, Patrick Hole, Rebecca Dragovic
      Pages 153-173
    5. Virginia Camacho, Vasilis Toxavidis, John C. Tigges
      Pages 175-190
    6. Veronika Lukacs-Kornek, Henrike Julich-Haertel, Sabine Katharina Urban, Miroslaw Kornek
      Pages 201-208
    7. Takanori Akagi, Takanori Ichiki
      Pages 209-217
  7. Imaging of Extracellular Vesicles

    1. Front Matter
      Pages 219-219
    2. Dmitry Ter-Ovanesyan, Emma J. K. Kowal, Aviv Regev, George M. Church, Emanuele Cocucci
      Pages 233-241
  8. Labeling and Tracking of Extracellular Vesicles

    1. Front Matter
      Pages 243-243
    2. Anthony Yan-Tang Wu, Charles Pin-Kuang Lai
      Pages 255-265
  9. Downstream Extracellular Vesicle Applications: Genomics and Proteomics

  10. Isolation of Extracellular Vesicles from Biofluids

    1. Front Matter
      Pages 319-319
    2. Shlomit Kenigsberg, Brandon A. Wyse, Clifford L. Librach, Juliano C. da Silveira
      Pages 321-341
    3. Kazuya Iwai, Satoshi Yamamoto, Mitsutaka Yoshida, Kiyotaka Shiba
      Pages 343-350
    4. Li-Guo Liang, Ye-Feng Sheng, Sherry Zhou, Fatih Inci, Lanjuan Li, Utkan Demirci et al.
      Pages 355-364
  11. Extracellular Vesicles Isolated from Cell Culture, Parasites and Stem Cells

    1. Front Matter
      Pages 365-365
    2. Chris Wang, Austin Wang, Fang Wei, David T. W. Wong, Michael Tu
      Pages 367-376
    3. Smart Mbagwu, Michael Walch, Luis Filgueira, Pierre-Yves Mantel
      Pages 377-388
    4. Zixin Chen, Yongjun Li, Hong Yu, Yan Shen, Chengwei Ju, Genshan Ma et al.
      Pages 389-394
  12. Extracellular Vesicles in Mouse Models

    1. Front Matter
      Pages 395-395
    2. Noemí García-Romero, Gorjana Rackov, Cristobal Belda-Iniesta, Ángel Ayuso-Sacido
      Pages 397-406
  13. Therapeutic Applications of Extracellular Vesicles

    1. Front Matter
      Pages 407-407
    2. Gareth R. Willis, Stella Kourembanas, S. Alex Mitsialis
      Pages 409-432
    3. Jing Liu, Jingwei Ma, Ke Tang, Bo Huang
      Pages 433-440
  14. Back Matter
    Pages 441-446

About this book


This volume examines established methods and protocols to isolate and characterize extracellular vesicles (EVs) and their composition, among other techniques including purification, imaging, biofluid-specific and cell-specific isolation and downstream genomic and proteomic profiling. The international group of expert scientists who have contributed to this collection provide a variety of different techniques related to the growing assortment of EV applications, given that at times using only one technique or two is insufficient to address the question at hand. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Extracellular Vesicles: Methods and Protocols serves as an ideal guide for researchers seeking to expand our knowledge of EV functions and applications.


Biogenesis Exosomes Microvesicles Inter-cellular communication EVs Isolation Precision medicine

Editors and affiliations

  • Winston Patrick Kuo
    • 1
  • Shidong Jia
    • 2
  1. 1.CloudHealth Genomics, Ltd.ShanghaiChina
  2. 2.Predicine, Inc.HaywardUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7251-7
  • Online ISBN 978-1-4939-7253-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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