Mammalian Synthetic Promoters

  • David Gould

Part of the Methods in Molecular Biology book series (MIMB, volume 1651)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Monitoring Promoter Interactions and Activity

    1. Front Matter
      Pages 9-9
    2. Ghazal Lashgari, Rami S. Kantar, Bakhos A. Tannous
      Pages 33-47
    3. Juliette M. K. M. Delhove, Rajvinder Karda, Kate E. Hawkins, Lorna M. FitzPatrick, Simon N. Waddington, Tristan R. McKay
      Pages 49-64
    4. Taher E. I. Taher
      Pages 65-73
  3. Promoter Design and Applications

    1. Front Matter
      Pages 75-75
    2. Dan Y. Even, Adi Kedmi, Diana Ideses, Tamar Juven-Gershon
      Pages 77-91
    3. Michael L. Roberts, Polyxeni Katsoupi, Vivian Tseveleki, Era Taoufik
      Pages 93-112
    4. Maria Veronica Lopez, Eduardo G. Cafferata, Diego L. Viale, Osvaldo L. Podhajcer
      Pages 113-130
    5. Hodan Mohamed, David Gould
      Pages 147-156
  4. Regulated Promoter Approaches

    1. Front Matter
      Pages 157-157
    2. Nasim Yousaf, David Gould
      Pages 159-172
    3. Maximilian Hörner, Konrad Müller, Wilfried Weber
      Pages 173-186
    4. Ryohei Ogawa, Go Kagiya, Akihiko Watanabe, Akihiro Morii, Zheng-Guo Cui, Takashi Kondo
      Pages 187-203
    5. Abraham O. Rodríguez-De la Fuente, J. Antonio Heredia-Rojas, Juan M. Alcocer-González, Laura E. Rodríguez-Flores, Cristina Rodríguez-Padilla, Reyes S. Taméz-Guerra
      Pages 205-212
    6. Eduardo G. Cafferata, Maria Veronica Lopez, Felipe J. Nuñez, Maria A. R. Maenza, Osvaldo L. Podhajcer
      Pages 213-227
  5. Combining Promoters in Synthetic Circuits

    1. Front Matter
      Pages 229-229
    2. Yinqing Li, Ron Weiss
      Pages 231-248
    3. James T. MacDonald, Velia Siciliano
      Pages 249-262
    4. Pratik Saxena, Daniel Bojar, Martin Fussenegger
      Pages 263-273
  6. Back Matter
    Pages 275-277

About this book


This volume details methods that will aid in the selection of promoter sequences and vector components and methods for the assembly and testing of synthetic promoters with examples of their application. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mammalian Synthetic Promoters: Methods and Protocols aims to aid researchers that are new to the field of synthetic promoters and inspire new developments.


synthetic promoters gene therapy pharmacologically regulated expression promoter sequences vector components

Editors and affiliations

  • David Gould
    • 1
  1. 1.Department of Biochemical PharmacologyWilliam Harvey Research Institute, Queen Mary University of LondonLondonUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7221-0
  • Online ISBN 978-1-4939-7223-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors