mRNA Processing

Methods and Protocols

  • Yongsheng Shi

Part of the Methods in Molecular Biology book series (MIMB, volume 1648)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Eimile Oakes, Pranathi Vadlamani, Heather A. Hundley
    Pages 103-127
  3. Shayna Stein, Emad Bahrami-Samani, Yi Xing
    Pages 129-142
  4. Deirdre C. Tatomer, Dongming Liang, Jeremy E. Wilusz
    Pages 143-154
  5. Angela Garibaldi, Francisco Carranza, Klemens J. Hertel
    Pages 169-176
  6. Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt et al.
    Pages 177-200 Open Access
  7. Chao Feng, Dalen Chan, Robert C. Spitale
    Pages 247-256
  8. Eric L. Van Nostrand, Thai B. Nguyen, Chelsea Gelboin-Burkhart, Ruth Wang, Steven M. Blue, Gabriel A. Pratt et al.
    Pages E1-E1
  9. Back Matter
    Pages 257-258

About this book


This detailed volume assembles a number of the most commonly used and state-of-the-art methodologies in the revolutionary field of mRNA processing in order to aid researchers in choosing the best method for their specific problems. Given that the vast majority of alternative mRNA processing events has yet to be explored, these techniques promise to breathe new life into the myriad new ideas currently underway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Practical and easy to use, mRNA Processing: Methods and Protocols seeks to aid scientists venturing into what’s certain to be another explosive period of growth in this dynamic field of study.


Metabolic labeling Bioinformatic tools Splicing patterns Alternative polyadenylation Circular RNAs

Editors and affiliations

  • Yongsheng Shi
    • 1
  1. 1.Department of Microbiology and Molecular Genetics, School of MedicineUniversity of CaliforniaIrvineUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7203-6
  • Online ISBN 978-1-4939-7204-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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