Circulating Tumor Cells

Methods and Protocols

  • Mark Jesus M. Magbanua
  • John W. Park

Part of the Methods in Molecular Biology book series (MIMB, volume 1634)

Table of contents

  1. Front Matter
    Pages i-xxv
  2. Emily S. Park, Simon P. Duffy, Hongshen Ma
    Pages 21-32
  3. Jose I. Varillas, Kangfu Chen, Jinling Zhang, Thomas J. George Jr., Z. Hugh Fan
    Pages 33-53
  4. Priya Gogoi, Saedeh Sepehri, Will Chow, Kalyan Handique, Yixin Wang
    Pages 55-64
  5. Sijie Hao, Merisa Nisic, Hongzhang He, Yu-Chong Tai, Si-Yang Zheng
    Pages 81-91
  6. Siddarth Rawal, Zheng Ao, Ram H. Datar, Ashutosh Agarwal
    Pages 93-105
  7. Marianne Oulhen, Emma Pailler, Vincent Faugeroux, Françoise Farace
    Pages 133-141
  8. Chin Chun Ooi, Seung-min Park, Dawson J. Wong, Sanjiv S. Gambhir, Shan X. Wang
    Pages 153-162
  9. Arturo B. Ramirez, Lance U’Ren, Daniel E. Campton, David Stewart, Joshua J. Nordberg, Jackie L. Stilwell et al.
    Pages 163-172
  10. Jeffrey L. Werbin, Joshua J. Nordberg, Jay Tzucker, Paulina Varshavskaya, Jackie L. Stilwell, Eric P. Kaldjian
    Pages 173-180
  11. Jackie L. Stilwell, Paulina Varshavskaya, Jeffrey L. Werbin, Joshua J. Nordberg, Arturo B. Ramirez, Steve Quarre et al.
    Pages 181-192
  12. Mark Jesus M. Magbanua, Tulasi I. Solanki, Andrea D. Ordonez, Feng Hsiao, John W. Park
    Pages 203-210
  13. Tomoyuki Okumura, Tetsuji Yamaguchi, Toru Watanabe, Takuya Nagata, Yutaka Shimada
    Pages 211-217
  14. Brandon Miller, Maryam Lustberg, Thomas A. Summers, Jeffrey J. Chalmers
    Pages 219-234
  15. Xunbin Wei, Jian Zhou, Xi Zhu, Xinrong Yang, Ping Yang, Qiyan Wang
    Pages 247-262
  16. Alexandra Soler, Laure Cayrefourcq, Martine Mazel, Catherine Alix-Panabières
    Pages 263-276
  17. M. J. Alvarez Cubero, J. A. Lorente, I. Robles-Fernandez, A. Rodriguez-Martinez, J. L. Puche, M. J. Serrano
    Pages 283-303
  18. Back Matter
    Pages 305-306

About this book


This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Circula

ting Tumor Cells: Methods and Protocols is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs.


Cytometric imaging Expression microarray Next generation RNA Fluorescence-activated cells sorting Immunomagnetic CTCs

Editors and affiliations

  • Mark Jesus M. Magbanua
    • 1
  • John W. Park
    • 2
  1. 1.Division of Hematology Oncology, Helen Diller Family Comprehensive Cancer CenterUniversity of California-San FranciscoSan FranciscoUSA
  2. 2.Division of Hematology Oncology, Helen Diller Family Comprehensive Cancer CenterUniversity of California-San FranciscoSan FranciscoUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7143-5
  • Online ISBN 978-1-4939-7144-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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