Acute Myeloid Leukemia

Methods and Protocols

  • Paolo Fortina
  • Eric Londin
  • Jason Y. Park
  • Larry J. Kricka

Part of the Methods in Molecular Biology book series (MIMB, volume 1633)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Michele E. Paessler, Marybeth Helfrich, Gerald B. W. Wertheim
    Pages 19-32
  3. Michael W. Cruise
    Pages 33-49
  4. Weina Chen, Hung S. Luu
    Pages 51-73
  5. Zhihong Zeng, Marina Konopleva, Michael Andreeff
    Pages 75-86
  6. Marco Alessandrini, Sharon S. Kappala, Michael S. Pepper
    Pages 101-123
  7. Gerald B. W. Wertheim, Marlise R. Luskin, Martin Carroll, Stephen R. Master
    Pages 125-136
  8. Mira Jeong, Anna G. Guzman, Margaret A. Goodell
    Pages 137-149
  9. Jonathan Schumacher, Philippe Szankasi, Todd W. Kelley
    Pages 151-161
  10. Eric Konnick, Christina M. Lockwood, David Wu
    Pages 163-184
  11. Alvin C. H. Ma, Xiangguo Shi, Bai-Liang He, Yuhan Guo, Anskar Y. H. Leung
    Pages 193-218
  12. Patricia M. Jones, Richard Boriack, Eduard A. Struys, Dinesh Rakheja
    Pages 219-234
  13. Chang-Sook Hong, Sonja Funk, Theresa L. Whiteside
    Pages 257-265
  14. Back Matter
    Pages 277-278

About this book


This volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival. The chapters in this book cover topics such as: cytochemical staining, single-cell mass cytometry of AML and Leukemia stem/progenitor cells, microsphere-based assessment of DNA methylation for AML prognosis, a zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation, and the isolation of biologically active exosomes from plasma of patients with cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Acute Myeloid Leukemia: Methods and Protocols is a valuable resource for scientists and researchers to further their studies and advancements in the field of AML.


heterogeneous disease cytogenetics reciprocal translocations transcription factor genes tyrosine kinases mRNA expression profiling AML

Editors and affiliations

  • Paolo Fortina
    • 1
  • Eric Londin
    • 2
  • Jason Y. Park
    • 3
  • Larry J. Kricka
    • 4
  1. 1.Thomas Jefferson University, Sidney Kimmel Cancer CenterPhiladelphiaUSA
  2. 2.Department of Pathology, Anatomy and Cell BiologySidney Kimmel Medical College Thomas Jefferson UniversityPhiladelphiaUSA
  3. 3.Department of PathologyUT Southwestern Medical Center and Children’s Medical CenterDallasUSA
  4. 4.Department of Pathology and Laboratory MedicineUniversity of Pennsylvania Medical CenterPhiladelphiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7140-4
  • Online ISBN 978-1-4939-7142-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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