RNA Nanostructures

Methods and Protocols

  • Eckart Bindewald
  • Bruce A. Shapiro

Part of the Methods in Molecular Biology book series (MIMB, volume 1632)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Li-Zhen Sun, Shi-Jie Chen
    Pages 1-17
  3. Rishabh Sharan, Eckart Bindewald, Wojciech K. Kasprzak, Bruce A. Shapiro
    Pages 19-32
  4. Taejin Kim, Wojciech K. Kasprzak, Bruce A. Shapiro
    Pages 33-64
  5. Gandharva Nagpal, Kumardeep Chaudhary, Sandeep Kumar Dhanda, Gajendra Pal Singh Raghava
    Pages 75-90
  6. Maria L. Kireeva, Kirill A. Afonin, Bruce A. Shapiro, Mikhail Kashlev
    Pages 91-105
  7. Aleksandra P. Dabkowska, Agnes Michanek, Luc Jaeger, Arkadiusz Chworos, Tommy Nylander, Emma Sparr
    Pages 107-122
  8. Kheiria Benkato, Benjamin O’Brien, My N. Bui, Daniel L. Jasinski, Peixuan Guo, Emil F. Khisamutdinov
    Pages 123-133
  9. Mark A. Boerneke, Thomas Hermann
    Pages 135-149
  10. Ganesh L. Lokesh, Hongyu Wang, Curtis H. Lam, Varatharasa Thiviyanathan, Nancy Ward, David G. Gorenstein et al.
    Pages 151-174
  11. Merve Denizli, Burcu Aslan, Lingegowda S. Mangala, Dahai Jiang, Cristian Rodriguez-Aguayo, Gabriel Lopez-Berestein et al.
    Pages 219-230
  12. Anu Puri, Serena Zampino, Mathias Viard, Bruce A. Shapiro
    Pages 241-253
  13. Kai Tian, Ruicheng Shi, Amy Gu, Michael Pennella, Li-Qun Gu
    Pages 255-268
  14. Angelica N. Martins, Weina Ke, Vaishnavi Jawahar, Morriah Striplin, Caryn Striplin, Eric O. Freed et al.
    Pages 269-283
  15. Paul Zakrevsky, Lorena Parlea, Mathias Viard, Eckart Bindewald, Kirill A. Afonin, Bruce A. Shapiro
    Pages 303-324
  16. Takahiro Tanaka, Yoshiya Ikawa, Shigeyoshi Matsumura
    Pages 325-340
  17. Back Matter
    Pages 381-383

About this book


This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures. The chapters in this book cover topics such as ion effects in RNA folding; design and crystallography of self-assembling RNA nanostructures; x-aptamer selection and validation; RNAi in HIV-infected cells; and preparation of a conditional RNA switch. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, RNA Nanostructures: Methods and Protocols is a valuable resource for the design and production of RNA nanostructures. Researchers and scientists sharing these detailed protocols is important for sustained progress in the field.


RNA Architectonics 3D Structure Prediction Exosomes Peptides Chrystallography

Editors and affiliations

  • Eckart Bindewald
    • 1
  • Bruce A. Shapiro
    • 2
  1. 1.Frederick National Laboratory for Cancer ResearchLeidos Biomedical ResearchFrederickUSA
  2. 2.RNA Structure and Design Section, RNA Biology LaboratoryNational Cancer Institute, National Institutes of HealthFrederickUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7137-4
  • Online ISBN 978-1-4939-7138-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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