Methods and Protocols

  • Jeff Wilkesman
  • Liliana Kurz

Part of the Methods in Molecular Biology book series (MIMB, volume 1626)

Table of contents

  1. Front Matter
    Pages i-xx
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Jeff Wilkesman, Liliana Kurz
      Pages 3-10
  3. Endopeptidase Zymography

    1. Front Matter
      Pages 11-11
    2. Jeff Wilkesman
      Pages 25-31
    3. Handunge Kumudu Irani Perera
      Pages 43-52
    4. Péter Bencsik, Monika Bartekova, Anikó Görbe, Krisztina Kiss, János Pálóczi, Jana Radosinska et al.
      Pages 53-70
    5. Goran Mucić, Brankica Rašković, Natalija Polović
      Pages 71-78
    6. Zongli Ren, Juanjuan Chen, Raouf A. Khalil
      Pages 79-102
    7. Madathiparambil G. Madanan, Ambili Mechoor
      Pages 103-114
  4. Reverse Zymography and In Situ Zymography

    1. Front Matter
      Pages 123-123
    2. Kanika Sharma, Debasish Bhattacharyya
      Pages 125-132
    3. Aastha Chhabra, Vibha Rani
      Pages 133-143
  5. 2D Zymography

    1. Front Matter
      Pages 145-145
    2. Shanyan Chen, Fanjun Meng, Zhenzhou Chen, Zhe Qu, Jiankun Cui, Zezong Gu
      Pages 147-155
    3. Jeff Wilkesman, María Fernanda Padrón, Liliana Kurz, Hervé Rémignon
      Pages 157-166
  6. Special Zymography Cases

    1. Front Matter
      Pages 167-167
    2. Diana Castro, Lellys M. Contreras, Liliana Kurz, Jeff Wilkesman
      Pages 199-204
    3. Jéssyka Fernanda Santiago Monte, Cláudia Jassica Gonçalves Moreno, Joana Patrícia Molato Figueiredo Lopes Monteiro, Hugo Alexandre de Oliveira Rocha, Aline Rimoldi Ribeiro, Marcelo Sousa Silva
      Pages 213-220
    4. Ambili Mechoor, Madathiparambil G. Madanan
      Pages 221-228
    5. Bahar S. Razavi, Duyen Hoang, Yakov Kuzyakov
      Pages 229-238
    6. Daniel Pan, Karl A. Wilson, Anna Tan-Wilson
      Pages 253-269
    7. Liliana Kurz, Zully Hernández, Lellys M. Contreras, Jeff Wilkesman
      Pages 271-277
    8. María Tapizquent, Marleny Fernández, Georgina Barreto, Zully Hernández, Lellys M. Contreras, Liliana Kurz et al.
      Pages 295-300
    9. Adarelys Andrades, Lellys M. Contreras
      Pages 301-308
  7. Back Matter
    Pages 309-310

About this book


This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases. Written in the highly respected Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Zymography: Methods and Protocols serves as an ideal aid for a broad array of researchers from the fields of biological and medicinal science, as well as scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.


Enzyme activity Electrophoretic gels Endopeptidase zymography In situ zymography Isozymes and allozymes Enzymology Gene markers

Editors and affiliations

  • Jeff Wilkesman
    • 1
  • Liliana Kurz
    • 2
  1. 1.Centre for Environmental, Biology and Chemistry Research, Facultad de Ciencias y TecnologíaUniversity of CaraboboValenciaVenezuela
  2. 2.Centre for Environmental, Biology and Chemistry Research Facultad de Ciencias y TecnologíaUniversity of CaraboboValenciaVenezuela

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7109-1
  • Online ISBN 978-1-4939-7111-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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