Plant Receptor Kinases

Methods and Protocols

  • Reidunn Birgitta  Aalen

Part of the Methods in Molecular Biology book series (MIMB, volume 1621)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Expression and Purification of PRKs

    1. Front Matter
      Pages 1-1
    2. Moneeza Akbar Agha, David Lightfoot, Ahmed Jawaad Afzal
      Pages 3-20
    3. María Laura Barberini, Jorge P. Muschietti
      Pages 21-27
    4. Hidefumi Shinohara, Yoshikatsu Matsubayashi
      Pages 29-35
    5. Akira Nozawa, Keiichirou Nemoto, Shunsuke Nomura, Satoshi Yamanaka, Kohki Kido, Tatsuya Sawasaki
      Pages 37-46
  3. Ligand Binding and Activation of PRKs

    1. Front Matter
      Pages 57-57
    2. Hidefumi Shinohara, Yoshikatsu Matsubayashi
      Pages 59-68
  4. In silico Methods for Identification and Analyses of PRKs

    1. Front Matter
      Pages 77-77
    2. Aleksia Vaattovaara, Jarkko Salojärvi, Michael Wrzaczek
      Pages 79-91
  5. Identification of Enzymatic Functions and Interacting Partners of PRKs

    1. Front Matter
      Pages 107-107
    2. María Laura Barberini, Jorge P. Muschietti
      Pages 109-112
    3. Keiichirou Nemoto, Akira Nozawa, Satoshi Yamanaka, Shunsuke Nomura, Kohki Kido, Tatsuya Sawasaki
      Pages 113-120
    4. Cornelia Fischer, Margret Sauter, Petra Dietrich
      Pages 141-149
  6. Imaging of PRK Dynamics

    1. Front Matter
      Pages 161-161
    2. Stefanie Weidtkamp-Peters, Yvonne Stahl
      Pages 163-175
    3. Jenna Loiseau, Silke Robatzek
      Pages 177-189
  7. Back Matter
    Pages 191-196

About this book


This is volume presents protocols relevant for plant receptor kinases(PRK) both in development and defence. Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways. Written in the highly successful Methods

in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Receptor Kinases: Methods and Protocols aims to ensure successful results in the further study of this vital field.


plant receptor kinases eukaryote in planta yeast plasma membrane wheat germ cell-free expression photoaffinity labeling

Editors and affiliations

  • Reidunn Birgitta  Aalen
    • 1
  1. 1.Department of BiosciencesUniversity of OsloOsloNorway

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7062-9
  • Online ISBN 978-1-4939-7063-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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