Methods and Protocols

  • Lucília Domingues

Part of the Methods in Molecular Biology book series (MIMB, volume 1620)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Ruslan Kalendar, Alexandr Muterko, Malika Shamekova, Kabyl Zhambakin
    Pages 1-31
  3. Ruslan Kalendar, Timofey V. Tselykh, Bekbolat Khassenov, Erlan M. Ramanculov
    Pages 33-64
  4. Eng Wee Chua, Simran Maggo, Martin A. Kennedy
    Pages 65-74
  5. Diogo Silva, Gustavo Santos, Mário Barroca, Tony Collins
    Pages 87-100
  6. Tatiana Q. Aguiar, Carla Oliveira, Lucília Domingues
    Pages 101-112
  7. Ana Filipa Sequeira, Joana L. A. Brás, Vânia O. Fernandes, Catarina I. P. D. Guerreiro, Renaud Vincentelli, Carlos M. G. A. Fontes
    Pages 113-128
  8. Flávio Azevedo, Humberto Pereira, Björn Johansson
    Pages 129-139
  9. Luc Bissonnette, Andrée F. Maheux, Michel G. Bergeron
    Pages 141-151
  10. Germán Villamizar-Rodríguez, Felipe Lombó
    Pages 153-162
  11. Francisco J. Santaclara, Montserrat Espiñeira
    Pages 163-171
  12. Montserrat Espiñeira, Francisco J. Santaclara
    Pages 173-181
  13. Farina Mujeeb, Preeti Bajpai, Neelam Pathak, Smita Rastogi Verma
    Pages 195-211
  14. Sujal Phadke, Andreia Filipa Salvador, Joana Isabel Alves, Orianna Bretschger, Maria Madalena Alves, Maria Alcina Pereira
    Pages 225-248
  15. Tina Kollannoor Johny, Sarita Ganapathy Bhat
    Pages 249-265
  16. Leigh Burgoyne, Lin Y. Koh, David Catcheside
    Pages 267-280
  17. Back Matter
    Pages 281-282

About this book


This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field.  Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology,  and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.


Cloning ERIC-PCR BOX-PCR DGGE DNA Fingerprinting

Editors and affiliations

  • Lucília Domingues
    • 1
  1. 1.CEB—Centre of Biological EngineeringUniversity of MinhoBragaPortugal

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7059-9
  • Online ISBN 978-1-4939-7060-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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