Hemorrhagic Fever Viruses

Methods and Protocols

  • Maria S. Salvato

Part of the Methods in Molecular Biology book series (MIMB, volume 1604)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Surveillance, Diagnosis and Classification of Hemorrhagic Fever Viruses

    1. Front Matter
      Pages 1-1
    2. Jean-Paul Gonzalez, Marc Souris, Willy Valdivia-Granda
      Pages 3-31
    3. Francisco J. Díaz, Luis E. Paternina, Juan David Rodas
      Pages 33-41
    4. Maria S. Salvato, Igor S. Lukashevich, Sandra Medina-Moreno, Juan Carlos Zapata
      Pages 79-88
    5. Luís E. Paternina, Juan David Rodas
      Pages 89-100
    6. Juan David Rodas, Andrés F. Londoño, Sergio Solari
      Pages 101-109
  3. Structural Studies and Reverse Genetics of Hemorrhagic Fever Viruses

    1. Front Matter
      Pages 111-111
    2. María Guadalupe Martínez, María Belén Forlenza, Nélida A. Candurra, Sandra M. Cordo
      Pages 113-133
    3. Antonella Pasquato, Antonio Herrador Fernandez, Stefan Kunz
      Pages 135-155
    4. Junjie Shao, Xiaoying Liu, Yuying Liang, Hinh Ly
      Pages 169-178
    5. Xiaoxuan Qi, Wenjian Wang, Haohao Dong, Yuying Liang, Changjiang Dong, Hinh Ly
      Pages 179-188
    6. Michael E. Lindquist, Connie S. Schmaljohn
      Pages 201-207
    7. Ronald N. Harty
      Pages 209-215
    8. Thomas Hoenen
      Pages 237-245
    9. Rekha Dhanwani, Qinfeng Huang, Shuiyun Lan, Yanqing Zhou, Junjie Shao, Yuying Liang et al.
      Pages 247-253
  4. In Vivo Models of Hemorrhagic Fever Virus Infection

    1. Front Matter
      Pages 255-255
    2. Rosana Gonzalez-Quintial, Roberto Baccala
      Pages 257-267
    3. Gary Wong, Yuhai Bi, Gary Kobinger, George F. Gao, Xiangguo Qiu
      Pages 269-278
    4. Maria S. Salvato, Igor S. Lukashevich, Yida Yang, Sandra Medina-Moreno, Mahmoud Djavani, Joseph Bryant et al.
      Pages 279-290
  5. Immune Assays and Vaccine Production for Hemorrhagic Fever Viruses

    1. Front Matter
      Pages 303-303
    2. Ana María Ambrosio, Mauricio Andrés Mariani, Andrea Soledad Maiza, Graciela Susana Gamboa, Sebastián Edgardo Fossa, Alejando Javier Bottale
      Pages 305-329
    3. Meng Ling Moi, Tomohiko Takasaki, Ichiro Kurane
      Pages 331-337
    4. John J. Suschak, Connie S. Schmaljohn
      Pages 339-348
  6. Host Responses to Viral Hemorrhagic Fever

    1. Front Matter
      Pages 349-349
    2. Federico Giovannoni, Jose Rafael Peña Cárcamo, María Laura Morell, Sandra Myriam Cordo, Cybele C. García
      Pages 351-370
    3. Claudia S. Sepúlveda, Cybele C. García, Elsa B. Damonte
      Pages 371-392
    4. Sheli R. Radoshitzky, Veronica Soloveva, Dima Gharaibeh, Jens H. Kuhn, Sina Bavari
      Pages 393-403
    5. Melissa L. Tursiella, Shannon L. Taylor, Connie S. Schmaljohn
      Pages 405-417
  7. Back Matter
    Pages 419-425

About this book


This volume presents protocols that analyze and explore hemorrhagic fever viruses (HFV). This book is divided into 5 parts: Part I begins with an overview on predicting viral pandemics and then covers methods for surveillance, diagnosis, and classification of HFV. This includes an antibody capture method using Lassa virus antigens. Part II discusses structural studies and reverse genetics of HFV. The chapters in this part describe envelope glycoprotein membrane fusion studies, arenavirus nucleocapsid protein, and the use of virus-like-particles to study viral egress. Part III explores in vivo models of HFV infections, and contains chapters on murine, guinea pig, and primate models for HFV, and methods to obtain a subset of primary human liver cells that can be cultured long-term. Part IV looks into immune assays and vaccine production for HFV. The chapters in this section cover the attenuated vaccine for Argentine HFV, detecting virus-antibody immune complexes in secondary dengue infections, and DNA vaccination. Part V discusses host responses to viral hemorrhagic fever, and contains chapters on identifying host restrictions to Junín or Dengue infection, and a cell-culture method to assess coagulation after HFV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Hemorrhagic Fever Viruses: Methods and Protocols is a valuable resource for scientists and researchers who want to bridge the gap between virus recognition in surveillance and understanding host responses to infection.


HFV Zoonotic pathogens Vaccine development Phenotype identification Pathogenesis

Editors and affiliations

  • Maria S. Salvato
    • 1
  1. 1.School of MedicineUniversity of MarylandBaltimoreUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6980-7
  • Online ISBN 978-1-4939-6981-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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