Organ Regeneration

3D Stem Cell Culture & Manipulation

  • Takashi Tsuji

Part of the Methods in Molecular Biology book series (MIMB, volume 1597)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Atsushi Kuwahara, Tokushige Nakano, Mototsugu Eiraku
    Pages 17-29
  3. Keisuke Ishihara, Adrian Ranga, Matthias P. Lutolf, Elly M. Tanaka, Andrea Meinhardt
    Pages 43-55
  4. Chikafumi Ozone, Hidetaka Suga
    Pages 57-65
  5. Francesco Antonica, Dominika Figini Kasprzyk, Andrea Alex Schiavo, Mírian Romitti, Sabine Costagliola
    Pages 85-95
  6. Masamitsu Oshima, Miho Ogawa, Takashi Tsuji
    Pages 97-116
  7. Kyosuke Asakawa, Koh-ei Toyoshima, Takashi Tsuji
    Pages 117-134
  8. Miho Ogawa, Takashi Tsuji
    Pages 135-151
  9. Masatoshi Hirayama, Kazuo Tsubota, Takashi Tsuji
    Pages 153-165
  10. Yasuhiro Yoshimura, Atsuhiro Taguchi, Ryuichi Nishinakamura
    Pages 179-193
  11. Keisuke Sekine, Takanori Takebe, Hideki Taniguchi
    Pages 207-216
  12. Maxime M. Mahe, Nicole E. Brown, Holly M. Poling, Michael A. Helmrath
    Pages 229-245
  13. Maxime M. Mahe, Nicole E. Brown, Holly M. Poling, Michael A. Helmrath
    Pages E1-E1
  14. Back Matter
    Pages 247-248

About this book


This volume provides readers with a better understanding of organogenesis in developmental biology and next-generation organ regenerative therapy. This book focuses on recent studies of organ regeneration from stem cells using in vitro 3D cell culture and manipulation. The chapters cover topics such as generation of a 3D retinal tissue formation; functional salivary gland regeneration; liver regeneration using cultures liver bud; and in vivo model of small intestine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Organ Regeneration: 3D Stem Cell Culture and Manipulation is a valuable resource for scientists and researchers who are interested in this field.


Cerebellar Stem Cell Transplantation embryogenesis tissue replacement

Editors and affiliations

  • Takashi Tsuji
    • 1
  1. 1.Laboratory for Organ RegenerationRIKEN Center for Developmental BiologyKobeJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6947-0
  • Online ISBN 978-1-4939-6949-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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