Synthetic Protein Switches

Methods and Protocols

  • Viktor Stein

Part of the Methods in Molecular Biology book series (MIMB, volume 1596)

Table of contents

  1. Front Matter
    Pages i-xi
  2. General Strategies and Considerations

  3. Peptide Switches

    1. Front Matter
      Pages 57-57
    2. Alex Sternisha, Olga Makhlynets
      Pages 59-68
  4. Fluorescent and Bioluminescent Sensors

    1. Front Matter
      Pages 69-69
    2. Ben E. Clifton, Jason H. Whitfield, Inmaculada Sanchez-Romero, Michel K. Herde, Christian Henneberger, Harald Janovjak et al.
      Pages 71-87
    3. Joshua A. Mitchell, William H. Zhang, Michel K. Herde, Christian Henneberger, Harald Janovjak, Megan L. O’Mara et al.
      Pages 89-99
    4. Helen Farrants, Julien Hiblot, Rudolf Griss, Kai Johnsson
      Pages 101-117
    5. Shyam Nyati, Grant Young, Brian Dale Ross, Alnawaz Rehemtulla
      Pages 131-145
  5. β-lactamase Sensors

    1. Front Matter
      Pages 147-147
    2. Hui Chin Goh, Farid J. Ghadessy, Saurabh Nirantar
      Pages 167-177
  6. Proteolytic Sensors

    1. Front Matter
      Pages 195-195
    2. Viktor Stein, Kirill Alexandrov
      Pages 197-218
  7. Optogenetic Switches

    1. Front Matter
      Pages 239-239
    2. Wignand W. D. Mühlhäuser, Maximilian Hörner, Wilfried Weber, Gerald Radziwill
      Pages 257-270
    3. Cristian Cosentino, Laura Alberio, Gerhard Thiel, Anna Moroni
      Pages 271-285
    4. Robert Stabel, Birthe Stüven, Robert Ohlendorf, Andreas Möglich
      Pages 287-304
  8. Cellular Signaling Switches

    1. Front Matter
      Pages 305-305
    2. Javier Castillo-Montoya, Indraneel Ghosh
      Pages 307-319
    3. Raphaël B. Di Roberto, Benjamin M. Scott, Sergio G. Peisajovich
      Pages 321-337
  9. Back Matter
    Pages 339-341

About this book


This detailed book provides state-of-the-art protocols to facilitate the construction of synthetic protein switches for a variety of applications in biotechnology and basic research. Beginning with general strategies, the volume continues with coverage of peptide switches, fluorescent and bioluminescent sensors, β-lactamase sensors, proteolytic sensors, optogenetic switches, as well as cellular signaling switches.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Synthetic Protein Switches: Methods and Protocols serves as a useful reference for life scientists from diverse research fields that range from traditional, discovery-centered disciplines, like cancer research, to newly emerging disciplines, such as synthetic biology.


Bioluminescent sensors Fluorescent sensors Synthetic biology Next-generation therapeutics Peptide switches β-lactamase Optogenetic switches Biomolecular analytes

Editors and affiliations

  • Viktor Stein
    • 1
  1. 1.Fachbereich BiologieTechnische Universität DarmstadtDarmstadtGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6938-8
  • Online ISBN 978-1-4939-6940-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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