Food Allergens

Methods and Protocols

  • Jing Lin
  • Marcos Alcocer

Part of the Methods in Molecular Biology book series (MIMB, volume 1592)

Table of contents

  1. Front Matter
    Pages i-x
  2. Food Allergen Purification and Production

    1. Front Matter
      Pages 11-11
    2. Barbara Cases, Carlos Pastor-Vargas, Marina Perez-Gordo
      Pages 13-22
    3. Changqi Liu, LeAnna N. Willison, Shridhar K. Sathe
      Pages 23-45
    4. Maria Neophytou, Marcos Alcocer
      Pages 47-56
  3. Food Allergen Discovery, Detection, and Quantification

    1. Front Matter
      Pages 57-57
    2. Galina Grishina, Luda Bardina, Alexander Grishin
      Pages 59-69
    3. Fernando de la Cuesta, Gloria Alvarez-Llamas, Maria G. Barderas
      Pages 71-78
    4. George N. Konstantinou
      Pages 79-94
    5. Aina García, Raquel Madrid, Teresa García, Rosario Martín, Isabel González
      Pages 95-108
    6. Raquel Madrid, Silvia de la Cruz, Aina García, Rosario Martín, Isabel González, Teresa García
      Pages 109-128
    7. Nuzul N. Jambari, XiaoWei Wang, Marcos Alcocer
      Pages 129-137
    8. Madhan Masilamani, Mohanapriya Kamalakannan, Hugh A. Sampson
      Pages 139-146
  4. Allergenic Epitope Mapping

    1. Front Matter
      Pages 163-163
    2. Jing Lin, Hugh A. Sampson
      Pages 177-187
    3. Madhan Masilamani, Mariona Pascal, Hugh A. Sampson
      Pages 189-198
    4. George N. Konstantinou
      Pages 211-222
  5. Methods Currently Being Developed and Future Development

    1. Front Matter
      Pages 247-247
    2. Silvia de la Cruz, Inés López-Calleja, Rosario Martín, Isabel González, Marcos Alcocer, Teresa García
      Pages 263-295
  6. Back Matter
    Pages 297-299

About this book


This volume provides a collection of methodologies for basic research, clinical diagnosis, and treatment pertaining to food allergens, including food allergen production, purification, characterization, detection, quantification, and bioinformatics approaches to modern food allergen studies. The chapters in the book are divided into 4 parts: Part I discusses food allergen purification and production, and explores methods of producing recombinant food allergens in bacterial and yeast expression systems; Part II looks at allergen discovery, detection, and quantification covering 3 types of methods—DNA-, protein-, and cell-based methods; Part III focuses on allergenic epitope mapping; and Part IV talks about future developments concentrated around new concepts of allergenicity as an  outcome of protein and food matrix interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Food Allergens: Methods and Protocols is a valuable resource for immunologists, biochemists, molecular biologists, and medical doctors and students working in the food allergy field. This book is also useful for people in the food industry, legislators, food standard agencies, allergologists, pediatricians, and clinicians in the allergic diseases and immunology fields.



ELISA PCR Detection Spectroscopy food science t-cell western blotting

Editors and affiliations

  • Jing Lin
    • 1
  • Marcos Alcocer
    • 2
  1. 1.Bioinformatics InstituteA*STARSingaporeSingapore
  2. 2.School of BiosciencesUniversity of Nottingham, Sutton Bonington CampusLeicestershireUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6923-4
  • Online ISBN 978-1-4939-6925-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals