Stem Cell Banking

Concepts and Protocols

  • Jeremy M. Crook
  • Tenneille E. Ludwig

Part of the Methods in Molecular Biology book series (MIMB, volume 1590)

Table of contents

  1. Front Matter
    Pages i-x
  2. Generic Themes in Stem Cell Banking

    1. Front Matter
      Pages 1-1
    2. Glyn Stacey
      Pages 3-10
    3. Therése Kallur, Pontus Blomberg, Sonya Stenfelt, Kristian Tryggvason, Outi Hovatta
      Pages 11-16
    4. Alberto Labarga, Izaskun Beloqui, Angel G. Martin
      Pages 29-39
    5. Kevin W. Bruce, John D. M. Campbell, Paul De Sousa
      Pages 79-98
    6. Donald Chalmers, Peter Rathjen, Joy Rathjen, Dianne Nicol
      Pages 99-112
  3. Protocols for Pluripotent Stem Cell Banking

    1. Front Matter
      Pages 113-113
    2. Jeremy M. Crook, Lucy Kravets, Teija Peura, Meri T. Firpo
      Pages 115-129
    3. Jennifer L. Brehm, Tenneille E. Ludwig
      Pages 139-150
    4. Jeremy M. Crook, Eva Tomaskovic-Crook, Tenneille E. Ludwig
      Pages 151-164
    5. Jared Carlson-Stevermer, Krishanu Saha
      Pages 165-174
  4. Protocols for Mesenchymal Stem Cell Banking

    1. Front Matter
      Pages 175-175
    2. Izaskun Ferrin, Izaskun Beloqui, Lorea Zabaleta, Juan M. Salcedo, Cesar Trigueros, Angel G. Martin
      Pages 177-190
    3. Andrés Pavón, Izaskun Beloqui, Juan M. Salcedo, Angel G. Martin
      Pages 191-196
  5. Protocols for Human Neural Stem Cell Banking

    1. Front Matter
      Pages 197-197
    2. Jeremy M. Crook, Eva Tomaskovic-Crook
      Pages 199-206
  6. Back Matter
    Pages 207-210

About this book


This volume covers protocols related to both pluripotent and somatic stem cells, including the ethical procurement of tissues and cells for the provision of “seed stock,” standardized methods for deriving hESCs and iPSCs, isolating mesenchymal stem cells, cell culture and cryopreservation, in addition to quality assurance and information management. Stem Cell Banking: Concepts and Protocols aims to contribute to the development of this field by providing information that is essential to establishing a bona fide stem cell bank. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Stem Cell Banking: Concepts and Protocols is a valuable resource for stem cell scientists and novices to the field, and will help strengthen and maximize their use of existing and future stem cell resources.



induced pluripotent stem cells (iPSCs) pluripotent and somatic stem cells seed stock hESCs mesenchymal stem cells cell culture and cryopreservation cell line characterization

Editors and affiliations

  • Jeremy M. Crook
    • 1
  • Tenneille E. Ludwig
    • 2
  1. 1.ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research InstituteAIIM Facility, Innovation Campus, University of Wollongong, Squires WayFairy MeadowAustralia
  2. 2.WiCell Research InstituteMadisonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6919-7
  • Online ISBN 978-1-4939-6921-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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