Heterologous Gene Expression in E.coli

Methods and Protocols

  • Nicola A. Burgess-Brown

Part of the Methods in Molecular Biology book series (MIMB, volume 1586)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. High-Throughput Cloning, Expression Screening, and Optimization

    1. Front Matter
      Pages 1-1
    2. Yoav Peleg, Vadivel Prabahar, Dominika Bednarczyk, Tamar Unger
      Pages 33-43
    3. Rosa Morra, Kate Young, David Casas-Mao, Neil Dixon, Louise E. Bird
      Pages 83-107
    4. Grietje Kuipers, Markus Peschke, Nurzian Bernsel Ismail, Anna Hjelm, Susan Schlegel, David Vikström et al.
      Pages 109-126
    5. Kaisa Ukkonen, Antje Neubauer, Vinit J. Pereira, Antti Vasala
      Pages 127-137
  3. Protein Purification and Solubility Enhancement

    1. Front Matter
      Pages 139-139
    2. Natalie J. Saez, Ben Cristofori-Armstrong, Raveendra Anangi, Glenn F. King
      Pages 155-180
    3. Ekaitz Errasti-Murugarren, Arturo Rodríguez-Banqueri, José Luis Vázquez-Ibar
      Pages 181-195
    4. Joshua Britton, Joshua N. Smith, Colin L. Raston, Gregory A. Weiss
      Pages 211-220
    5. Sreejith Raran-Kurussi, Scott Cherry, Di Zhang, David S. Waugh
      Pages 221-230
  4. Case Studies to Produce Challenging Proteins and Specific Protein Families

    1. Front Matter
      Pages 231-231
    2. Benjamin Strutton, Stephen R. P. Jaffé, Jagroop Pandhal, Phillip C. Wright
      Pages 233-250
    3. Charlotte A. Dodson
      Pages 251-264
    4. Imre Berger, Quiyang Jiang, Ryan J. Schulze, Ian Collinson, Christiane Schaffitzel
      Pages 279-290
    5. Ralf-Bernhardt Rues, Alexander Gräwe, Erik Henrich, Frank Bernhard
      Pages 291-312
    6. Katharina Karste, Maren Bleckmann, Joop van den Heuvel
      Pages 313-324
    7. Trang Phan, Phuong Huynh, Tuom Truong, Hoang Nguyen
      Pages 325-334
  5. Applications of E. coli Expression

    1. Front Matter
      Pages 335-335
    2. Susanne Gräslund, Pavel Savitsky, Susanne Müller-Knapp
      Pages 337-344
    3. Toshihiko Sugiki, Toshimichi Fujiwara, Chojiro Kojima
      Pages 345-357
    4. Charikleia Black, John J. Barker, Richard B. Hitchman, Hok Sau Kwong, Sam Festenstein, Thomas B. Acton
      Pages 359-371
    5. Rod Chalk
      Pages 373-395
    6. Stephen Wallace, Emily P. Balskus
      Pages 409-421
  6. Back Matter
    Pages 423-429

About this book


This detailed volume provides a toolbox for designing constructs, tackling expression and solubility issues, handling membrane proteins and protein complexes, and exploring innovative engineering of E. coli. The topics are largely grouped under four parts: high-throughput  cloning, expression screening, and optimization of expression conditions, protein production and solubility enhancement, case studies to produce challenging proteins and specific protein families, as well as applications of E. coli expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Heterologous Gene Expression in E. coli: Methods and Protocols serves molecular biologists, biochemists and structural biologists, those in the beginning of their research careers to those in their prime, to give both an historical and modern overview of the methods available to express their genes of interest in this exceptional organism.


Protein purification Recombinant proteins Escherichia coli Structural biology High-throughput cloning Expression screening Solubility enhancement

Editors and affiliations

  • Nicola A. Burgess-Brown
    • 1
  1. 1.SGC, Nuffield Department of Clinical MedicineUniversity of OxfordOxfordUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6885-5
  • Online ISBN 978-1-4939-6887-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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