Recombinant Virus Vaccines

Methods and Protocols

  • Maureen C. Ferran
  • Gary R. Skuse

Part of the Methods in Molecular Biology book series (MIMB, volume 1581)

Table of contents

  1. Front Matter
    Pages i-x
  2. Double-Stranded DNA Viruses

    1. Front Matter
      Pages 1-1
    2. Débora Garanzini, María Paula Del Médico-Zajac, Gabriela Calamante
      Pages 15-28
    3. Juan C. Corredor, Yanlong Pei, Éva Nagy
      Pages 29-54
    4. Richard Voellmy, David C. Bloom, Nuria Vilaboa, Joyce Feller
      Pages 55-78
    5. Feifei Tan, Xiangdong Li, Kegong Tian
      Pages 79-96
    6. Vincent Pavot, Sarah Sebastian, Alison V. Turner, Jake Matthews, Sarah C. Gilbert
      Pages 97-119
  3. Negative Sense Single-Stranded RNA Viruses

    1. Front Matter
      Pages 131-131
    2. Luis Martínez-Sobrido, Juan Carlos de la Torre
      Pages 133-149
    3. Michael D. Mühlebach, Stefan Hutzler
      Pages 151-168
    4. Rekha Dhanwani, Hinh Ly, Yuying Liang
      Pages 169-179
    5. Khamis Tomusange, Danushka Wijesundara, Eric James Gowans, Branka Grubor-Bauk
      Pages 181-201
  4. Positive Sense Single-Stranded RNA Viruses

    1. Front Matter
      Pages 223-223
    2. Kenneth Lundstrom
      Pages 225-242
  5. Bacteriophage

    1. Front Matter
      Pages 243-243
    2. Jonelle L. Mattiacio, Matt Brewer, Stephen Dewhurst
      Pages 245-253
  6. Back Matter
    Pages 269-273

About this book


This volume provides readers with methods and protocols for understanding the development of recombinant viruses and their use as vaccines platforms. Recombinant Virus Vaccines: Methods and Protocols details the use of recombinant vaccines that are employed to either produce immunogens in vitro or elicit antibody production in vivo. The chapters in this book are divided into four parts: Part I explores double-stranded DNA viruses; Part II discusses negative sense single-stranded RNA viruses; Part III talks about positive sense single-stranded RNA viruses; and Part IV describes bacteriophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Recombinant Virus Vaccines: Methods and Protocols is a valuable resource for scientists and clinicians who are interested in learning about and adopting methods for use in basic and biomedical research directed toward generating and developing recombinant viral vaccines.



Parvovirus antibody immunogens viral genome viral vaccines infection

Editors and affiliations

  • Maureen C. Ferran
    • 1
  • Gary R. Skuse
    • 2
  1. 1.Rochester Institute of TechnologyThomas H. Gosnell School of Life SciencesRochesterUSA
  2. 2.Rochester Institute of TechnologyThomas H. Gosnell School of Life SciencesRochesterUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6867-1
  • Online ISBN 978-1-4939-6869-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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