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Synthetic Antibodies

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Thomas Tiller

    1. MorphoSys AG, Discovery Alliances & Technologies, Planegg, Germany

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  • Includes cutting-edge methods and protocols for the study of synthetic antibodies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1575)

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Table of contents (27 protocols)

  1. Nucleotide-Based Synthetic Antibodies: Aptamers

    1. An Enzyme-Linked Aptamer Sorbent Assay to Evaluate Aptamer Binding

      • Matthew D. Moore, Blanca I. Escudero-Abarca, Lee-Ann Jaykus
      Pages 291-302

    2. Incorporating Aptamers in the Multiple Analyte Profiling Assays (xMAP): Detection of C-Reactive Protein

      • Elyse D. Bernard, Kathy C. Nguyen, Maria C. DeRosa, Azam F. Tayabali, Rocio Aranda-Rodriguez
      Pages 303-322

  2. Moleculary Imprinted Polymers

    1. Front Matter

      Pages 323-323
      Download chapter PDF

    2. Transferring the Selectivity of a Natural Antibody into a Molecularly Imprinted Polymer

      • Romana Schirhagl
      Pages 325-340

    3. Preparation of Molecularly Imprinted Microspheres by Precipitation Polymerization

      • Tibor Renkecz, Viola Horvath
      Pages 341-352

    4. Generation of Janus Molecularly Imprinted Polymer Particles

      • Xiantao Shen, Chuixiu Huang, Lei Ye
      Pages 353-362

    5. Surface Engineering of Nanoparticles to Create Synthetic Antibodies

      • Linda Chio, Darwin Yang, Markita Landry
      Pages 363-380

    6. H5N1 Virus Plastic Antibody Based on Molecularly Imprinted Polymers

      • Chak Sangma, Peter A. Lieberzeit, Wannisa Sukjee
      Pages 381-388

    7. Replacement of Antibodies in Pseudo-ELISAs: Molecularly Imprinted Nanoparticles for Vancomycin Detection

      • Francesco Canfarotta, Katarzyna Smolinska-Kempisty, Sergey Piletsky
      Pages 389-398

    8. Cell and Tissue Imaging with Molecularly Imprinted Polymers

      • Maria Panagiotopoulou, Stephanie Kunath, Karsten Haupt, Bernadette Tse Sum Bui
      Pages 399-415

  3. Erratum

    • Thomas Tiller
    Pages E1-E1

  4. Back Matter

    Pages 417-420
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Keywords

About this book

This detailed volume presents a set of protocols useful for researchers in the field of recombinant immunoglobulin and alternative scaffold engineering, aptamer development, and generation of molecularly imprinted polymers (MIPs). Part I includes methods that deal with amino-acid based synthetic antibodies. Brief protocols about the generation of antibody libraries are detailed, as well as techniques for antibody selection, characterization, and validation. This section is completed by a brief description of a bioinformatics platform that supports antibody engineering during research and development. Part II contains basic procedures about the selection and characterization of aptamer molecules, and Part III describes fundamental processes of MIP generation and application. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Synthetic Antibodies: Methods and Protocols is an ideal guide for scientists seeking to propel the vital study of antibody research.


Editors and Affiliations

  • MorphoSys AG, Discovery Alliances & Technologies, Planegg, Germany

    Thomas Tiller

Bibliographic Information

  • Book Title: Synthetic Antibodies

  • Book Subtitle: Methods and Protocols

  • Editors: Thomas Tiller

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6857-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media LLC 2017

  • Hardcover ISBN: 978-1-4939-6855-8Published: 05 March 2017

  • Softcover ISBN: 978-1-4939-8319-3Published: 20 June 2018

  • eBook ISBN: 978-1-4939-6857-2Published: 01 March 2017

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 420

  • Number of Illustrations: 28 b/w illustrations, 74 illustrations in colour

  • Topics: Immunology

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Health & Hospitals, Pharma

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