Histochemistry of Single Molecules

Methods and Protocols

  • Carlo Pellicciari
  • Marco Biggiogera

Part of the Methods in Molecular Biology book series (MIMB, volume 1560)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Vital Histochemistry

  3. Lectin Histochemistry

    1. Front Matter
      Pages 91-91
    2. Gerrit Wolters-Eisfeld, Udo Schumacher
      Pages 121-132
    3. Vicente Seco-Rovira, Ester Beltrán-Frutos, Jesús Martínez-Hernández, Concepción Ferrer, Luis Miguel Pastor
      Pages 133-144
  4. Protein Histochemistry

  5. Lipid Histochemistry

    1. Front Matter
      Pages 195-195
    2. Víctor Carriel, Fernando Campos, José Aneiros-Fernández, John A. Kiernan
      Pages 197-206
    3. Víctor Carriel, Antonio Campos, Miguel Alaminos, Stefania Raimondo, Stefano Geuna
      Pages 207-218
    4. Kerry Ann Rostron, Clare Louise Lawrence
      Pages 219-229
    5. Bo-Hua Chen, Huei-Jiun Yang, He-Yen Chou, Guang-Chao Chen, Wei Yuan Yang
      Pages 231-236
  6. Nuclear Histochemistry

    1. Front Matter
      Pages 237-237
    2. Giuliano Mazzini, Marco Danova
      Pages 239-259
    3. Irene Masiello, Marco Biggiogera
      Pages 261-267
    4. Nicolas Thelen, Marc Thiry
      Pages 269-276
    5. Marco Biggiogera, Irene Masiello
      Pages 277-283
    6. Elva I. Cortés-Gutiérrez, José Luis Fernández, Martha I. Dávila-Rodríguez, Carmen López-Fernández, Jaime Gosálvez
      Pages 285-293
  7. Plant Histochemistry

    1. Front Matter
      Pages 295-295
    2. Guillermo Laguna-Hernández, Alicia Enriqueta Brechú-Franco, Iván De la Cruz-Chacón, Alma Rosa González-Esquinca
      Pages 331-338
  8. Vicente Seco-Rovira, Ester Beltrán-Frutos, Jesús Martínez-Hernández, Concepción Ferrer, Luis Miguel Pastor
    Pages E1-E1
  9. Back Matter
    Pages 339-341

About this book


This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets.  It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital histochemistry, lectin histochemistry, and DNA  fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Histochemistry of Single Molecules: Methods and Protocols aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ.


Histology Nucleic acid detection Metabolic processes Optical and electron microscopy Histochemical techniques Chemical targets Cytochemical methods

Editors and affiliations

  • Carlo Pellicciari
    • 1
  • Marco Biggiogera
    • 2
  1. 1.Department of Biology and BiotechnologyUniversity of PaviaPaviaItaly
  2. 2.Department of Biology and BiotechnologyUniversity of PaviaPaviaItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6787-2
  • Online ISBN 978-1-4939-6788-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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