Signal Transduction Immunohistochemistry

Methods and Protocols

  • Alexander E. Kalyuzhny

Part of the Methods in Molecular Biology book series (MIMB, volume 1554)

Table of contents

  1. Front Matter
    Pages i-x
  2. Kathy Brumbaugh, Wen-Chie Liao, J. P. Houchins, Jeff Cooper, Steve Stoesz
    Pages 1-40
  3. Kameron Simpson, Michael Browning
    Pages 41-59
  4. Christianne Bandeira-Melo, Ligia Almeida Paiva, Natália R. T. Amorim, Peter F. Weller, Patricia T. Bozza
    Pages 127-141
  5. Charles A. Warwick, Yuriy M. Usachev
    Pages 161-173
  6. Paul Hauser, Sha Wang, Vladimir V. Didenko
    Pages 193-200
  7. Pravin J. Mishra, Debabrata Banerjee
    Pages 201-209
  8. David Schwartz, Michael Grahek, Yingwei He, Wei Wang, Jennifer Nguyen, Alexander E. Kalyuzhny
    Pages 219-227
  9. Jessica Kalra, Jennifer Baker
    Pages 237-251
  10. Jacob Ricca, Mesruh Turkekul, Afsar Barlas, Dmitry Yarilin, Sho Fujisawa, Ning Fan et al.
    Pages 253-262
  11. Mesruh Turkekul, Afsar Barlas, Dmitry Yarilin, Sho Fujisawa, Ning Fan, Matthew Brendel et al.
    Pages 263-272
  12. Nate Hopp, Jodi Hagen, Birte Aggeler, Alexander E. Kalyuzhny
    Pages 273-283
  13. Back Matter
    Pages 285-290

About this book


This second edition volume provides detailed protocols that address the challenges of signal-transduction IHC. This book delves into chapters that discuss the nature of signal transduction phenomena and approaches to making phosphor-specific antibodies, as well as numerous bona fide methods methods on digital imaging techniques, preservation of tissue targets, multicolor detection, flow cytometry, lipophagy analysis, apoptosis, and the combination of IHC with in situ hybridization. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and comprehensive, Signal Transduction Immunohistochemistry: Methods and Protocols, Second Edition is a valuable resource to both novices and experts in other fields of biomedical research who need advice on IHC protocols to study signal transduction. This book will also be useful for researchers in academia, government labs, and the biotech industry.


Cell Preservation ELISA antibodies chromogenesis post translation preservation of tissue targets flow cytometry multicolor detection stem cells lysosomal cell death IHC apoptosis

Editors and affiliations

  • Alexander E. Kalyuzhny
    • 1
  1. 1.Immunocytochemistry & Elispot AssaysBio-Techne, Inc.MinneapolisUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6757-5
  • Online ISBN 978-1-4939-6759-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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