About this book
This practical collection examines methodologies originating from the benefits of genome-wide approaches to studying epigenetics, which has opened the emerging field of epigenomics. Focusing on the areas of cancer, inflammatory and autoimmune disorders, chapters discuss three main components of the epigenome and their role in the regulation of gene expression and present a detailed method section specific to studying each component, including data analyses, troubleshooting, and feasibility in different experimental settings. The main topics are high-throughput and targeted methods for DNA methylation analysis, nucleosome position mapping, studying epigenetic effects of gut microbiota, optical imaging for detection of epigenetic aberrations in living cells, methods for microRNA, and histone code profiling. Written for the Methods in Pharmacology and Toxicologyseries, the book includes the kind of detail and implementation advice to encourage success in the lab.
Authoritative and easily applicable, Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases aims to provide pharmacologists, molecular biologists, bioinformaticians, and toxicologists with a vital background on epigenetics and state-of-the-art techniques in epigenomics.
DNA methylation Histone covalent modifications Non-coding RNA mechanisms Epigenomics Nucleosome position mapping Optical imaging Epigenetic aberrations
Editors and affiliations
- DOI https://doi.org/10.1007/978-1-4939-6743-8
- Copyright Information Springer Science+Business Media LLC 2017
- Publisher Name Humana Press, New York, NY
- eBook Packages Springer Protocols
- Print ISBN 978-1-4939-6741-4
- Online ISBN 978-1-4939-6743-8
- Series Print ISSN 1557-2153
- Series Online ISSN 1940-6053
- Buy this book on publisher's site