Exosomes and Microvesicles

Methods and Protocols

  • Andrew F Hill

Part of the Methods in Molecular Biology book series (MIMB, volume 1545)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Bernd Giebel, Clemens Helmbrecht
    Pages 1-20
  3. Sybren L. N. Maas, Marike L. D. Broekman, Jeroen de Vrij
    Pages 21-33
  4. Kym McNicholas, Michael Z. Michael
    Pages 35-42
  5. Romain Linares, Sisareuth Tan, Céline Gounou, Alain R. Brisson
    Pages 43-54
  6. Shayne A. Bellingham, Mitch Shambrook, Andrew F. Hill
    Pages 55-70
  7. Karine Laulagnier, Charlotte Javalet, Fiona J. Hemming, Rémy Sadoul
    Pages 129-138
  8. Rocío Pérez-González, Sebastien A. Gauthier, Asok Kumar, Mitsuo Saito, Mariko Saito, Efrat Levy
    Pages 139-151
  9. Pascal Leblanc, Zaira E. Arellano-Anaya, Emilien Bernard, Laure Gallay, Monique Provansal, Sylvain Lehmann et al.
    Pages 153-176
  10. Maria Aatonen, Sami Valkonen, Anita Böing, Yuana Yuana, Rienk Nieuwland, Pia Siljander
    Pages 177-188
  11. Shivakumar Keerthikumar, Lahiru Gangoda, Yong Song Gho, Suresh Mathivanan
    Pages 189-196
  12. Pieter Vader, Imre Mäger, Yi Lee, Joel Z. Nordin, Samir E. L. Andaloussi, Matthew J. A. Wood
    Pages 197-204
  13. Susan M. van Dommelen, Margaret Fish, Arjan D. Barendrecht, Raymond M. Schiffelers, Omolola Eniola-Adefeso, Pieter Vader
    Pages 205-213
  14. Aizea Morales-Kastresana, Jennifer C. Jones
    Pages 215-225
  15. Back Matter
    Pages 227-228

About this book

Introduction

This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. The chapters in this book discuss EV cargoes containing proteins and genomic materials using a number of different approaches, and isolating EVs from platelets and neuronal cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and comprehensive, Exosomes and Microvesicles: Methods and Protocols is a valuable resource containing methodologies for anyone interested in researching EVs.

Keywords

extracellular vesicles cellular biology cell-cell communication electron microscopy tunable resistance pulse sensing nanoparticle tracking analysis platelets neuronal cells and tissues neuroscience

Editors and affiliations

  • Andrew F Hill
    • 1
  1. 1.Department of Biochemistry and GeneticsLa Trobe Institute for Molecular Science, La Trobe UniversityBundooraAustralia

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6728-5
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6726-1
  • Online ISBN 978-1-4939-6728-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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