Promoter Associated RNA

Methods and Protocols

  • Sara Napoli

Part of the Methods in Molecular Biology book series (MIMB, volume 1543)

Table of contents

  1. Front Matter
    Pages i-x
  2. Identification of Promoter-Associated RNAs

  3. Characterization of Promoter-Associated RNA Features

    1. Front Matter
      Pages 127-127
    2. Chiara Pastori, Dmitry Velmeshev, Veronica Julia Peschansky
      Pages 129-143
    3. Fariza Tahi, Van Du T. Tran, Anouar Boucheham
      Pages 145-168
    4. Carla M. Mann, Usha K. Muppirala, Drena Dobbs
      Pages 169-185
    5. Ranveer Singh Jayani, Amanjot Singh, Dimple Notani
      Pages 187-193
  4. Functional Studies of Promoter-Associated RNAs

    1. Front Matter
      Pages 195-195
    2. Marta Soler, Raquel Boque-Sastre, Sonia Guil
      Pages 221-229
    3. Raquel Boque-Sastre, Marta Soler, Sonia Guil
      Pages 231-242
  5. Therapeutic Potential of Promoter-Associated RNAs Targeting

  6. Stéphane Poulain, Sachi Kato, Ophélie Arnaud, Jean-Étienne Morlighem, Makoto Suzuki, Charles Plessy et al.
    Pages E1-E1
  7. Back Matter
    Pages 283-287

About this book


This volume is divided in four sections; covering genome wide approaches, techniques for characterize of paRNA structural features are described, selecting pa-RNA, and paRNA therapeutic potential. Chapters describe how siRNAsdirected against paRNAs can be applied in vivo to modulate transcription of important genes controlled by paRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Promoter Associated RNA: Methods and Protocols aims to demonstrate paRNAs as new class of regulatory molecules, to further investigate and value as tools for fine transcriptional tuning.


paRNA promoter-associated RNA noncoding transcriptome noncoding RNA RNA binding proteins R-loop structures

Editors and affiliations

  • Sara Napoli
    • 1
  1. 1.Experimental Therapeutics GroupInstitute of Oncology ResearchBellinzonaSwitzerland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6714-8
  • Online ISBN 978-1-4939-6716-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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