Cancer Cytogenetics

Methods and Protocols

  • Thomas S.K. Wan

Part of the Methods in Molecular Biology book series (MIMB, volume 1541)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Thomas S. K. Wan
    Pages 1-10
  3. Eleanor K. C. Hui, Thomas S. K. Wan, Margaret H. L. Ng
    Pages 11-17
  4. Dorota Koczkodaj, Agata A. Filip
    Pages 33-41
  5. Roderick A. F. MacLeod, Maren E. Kaufmann, Hans G. Drexler
    Pages 43-58
  6. Huifang Huang, Jiadi Chen
    Pages 59-66
  7. Thomas S. K. Wan, Eleanor K. C. Hui, Margaret H. L. Ng
    Pages 67-74
  8. Doron Tolomeo, Roscoe R. Stanyon, Mariano Rocchi
    Pages 91-100
  9. Jun Gu, Janice L. Smith, Patricia K. Dowling
    Pages 101-118
  10. Alvin S. T. Lim, Tse Hui Lim
    Pages 119-125
  11. Christine J. Ye, Henry H. Heng
    Pages 151-166
  12. Chengsheng Zhang, Eliza Cerveira, Mallory Romanovitch, Qihui Zhu
    Pages 167-179
  13. Thomas Liehr, Moneeb A. K. Othman, Katharina Rittscher
    Pages 181-187
  14. Michelle Ricoul, Tamizh Gnana-Sekaran, Laure Piqueret-Stephan, Laure Sabatier
    Pages 189-208
  15. John J. Yang, Tae Sung Park, Thomas S. K. Wan
    Pages 223-245

About this book

Introduction

This volume provides readers with detailed protocols covering the main cancer cytogenetics techniques needed for clinical utilization and research purposes. The chapters in this book cover topics such as chromosome preparation for myeloid malignancies; chromosome bandings; fluorescence in situ hybridization probe preparation; array-based comparative genomic hybridization; and cytogenetic nomenclature and reporting. The updated reviews on chromosomal abnormalities in hematological malignancies are excellent guides for cytogenetics data interpretations and specific malignant diseases correlation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and comprehensive, Cancer Cytogenetics: Methods and Protocols is a valuable resource for the novice in cytogenetics because it provides helpful guiding protocols, but it’s also great for those who are already engaged in the field and are looking for some technical hints.

Keywords

Array Comparative Genomic Hybridization (aCGH) FISH lymphoid myelodysplastic syndromes myeloid leukemia karyotyping chromosome bandings hybridization probe preparation data interpretations

Editors and affiliations

  • Thomas S.K. Wan
    • 1
  1. 1.Haematology Division Department of Anatomical and Cellular PathologyThe Chinese University of Hong Kong Prince of Wales HospitalShatin, Hong KongChina

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6703-2
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6701-8
  • Online ISBN 978-1-4939-6703-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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