Methods and Protocols

  • Wolfgang R. Streit
  • Rolf Daniel

Part of the Methods in Molecular Biology book series (MIMB, volume 1539)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Nancy Weiland-Bräuer, Daniela Langfeldt, Ruth A. Schmitz
    Pages 23-42
  3. Mikkel A. Glaring, Jan K. Vester, Peter Stougaard
    Pages 43-55
  4. Eleanor Jameson, Martin Taubert, Sara Coyotzi, Yin Chen, Özge Eyice, Hendrik Schäfer et al.
    Pages 57-74
  5. Bernd Wemheuer, Franziska Wemheuer
    Pages 75-84
  6. James Doonan, Sandra Denman, James E. McDonald, Peter N. Golyshin
    Pages 85-97
  7. Yuriy Rebets, Jan Kormanec, Andriy Luzhetskyy, Kristel Bernaerts, Jozef Anné
    Pages 99-144
  8. Rafael Bargiela, Manuel Ferrer
    Pages 145-157
  9. Nadine Katzke, Andreas Knapp, Anita Loeschcke, Thomas Drepper, Karl-Erich Jaeger
    Pages 159-196
  10. Dominique Böttcher, Patrick Zägel, Marlen Schmidt, Uwe T. Bornscheuer
    Pages 197-204
  11. Nele Ilmberger, Wolfgang R. Streit
    Pages 205-217
  12. Nele Ilmberger, Ulrich Rabausch
    Pages 229-236
  13. Jiujun Cheng, Ricardo Nordeste, Maria A. Trainer, Trevor C. Charles
    Pages 237-248
  14. Stéphane Uroz, Phil M. Oger
    Pages 271-286
  15. F. Jerry Reen, Jose A. Gutiérrez-Barranquero, Fergal O’Gara
    Pages 287-300
  16. Yuriy Rebets, Jan Kormanec, Andriy Luzhetskyy, Kristel Bernaerts, Jozef Anné
    Pages E1-E1
  17. Back Matter
    Pages 301-303

About this book


This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screening of libraries for diverse enzymes and biomolecules; current methods used to isolate DNA and construct large- and small-insert libraries from terrestrial and marine habitats; methods for establishing metagenome libraries in none-E.coli hosts; new molecular tools used for function-driven mining of metagenomic DNA; and screening protocols for a wide array of different genes encoding enzymes with relevance to biotechnology and ecology. Metagenomics: Methods and Protocols, Second Edition also provides detailed screening protocols for phosphatases, poly-hydroxyalkanoate, metabolism-related enzymes, stereoselective hydrolases, and microbial signals for the discovery of secondary metabolites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Metagenomics: Methods and Protocols, Second Edition is a collection of up-to-date metagenome protocols and tools for the recovery of numerous major types of biocatalysts, and allows researchers to easily setup these screens in any microbiology laboratory.



DNA isolation from soils and marine construction screening of libraries enzymes and biomolecules construct large- and small-insert libraries metagenome libraries in none-E. coli hosts function-driven mining of metagenomic DNA phosphatases metabolism-related enzymes microbial signals for discovery of secondary metabolites environmental genomics ecogenomics

Editors and affiliations

  • Wolfgang R. Streit
    • 1
  • Rolf Daniel
    • 2
  1. 1.Mikrobiologie & Biotechnologie Biocenter Klein FlottbekUniversity of HamburgHamburgGermany
  2. 2.Göttingen Genomics Laboratory Department of Genomic and Applied Microbiology Institut für Mikrobiologie und GenetikGeorg-August-Universität GöttingenGöttingenGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6689-9
  • Online ISBN 978-1-4939-6691-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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