Synapse Development

Methods and Protocols

  • Alexandros Poulopoulos

Part of the Methods in Molecular Biology book series (MIMB, volume 1538)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Forming Synapses in a Dish

    1. Front Matter
      Pages 1-1
    2. Katalin Czöndör, Olivier Thoumine
      Pages 29-44
    3. David P. Santos, Evangelos Kiskinis
      Pages 53-66
  3. Analyzing Synaptic Constituents

    1. Front Matter
      Pages 67-67
    2. Bekir Altas, Olaf Jahn, Hiroshi Kawabe
      Pages 69-82
    3. Nirmala Padmanabhan, Tabrez J. Siddiqui
      Pages 83-91
    4. Mary L. Michaelis, Lei Jiang, Elias K. Michaelis
      Pages 107-119
    5. Elisa Luquet, Christoph Biesemann, Annie Munier, Etienne Herzog
      Pages 121-134
  4. Observing Synaptic Structure and Ultrastructure

    1. Front Matter
      Pages 135-135
    2. Yoh Isogai, Douglas S. Richardson, Catherine Dulac, Joseph Bergan
      Pages 137-153
    3. Soyon Hong, Daniel K. Wilton, Beth Stevens, Douglas S. Richardson
      Pages 155-167
    4. Frank Herrmannsdörfer, Benjamin Flottmann, Siddarth Nanguneri, Varun Venkataramani, Heinz Horstmann, Thomas Kuner et al.
      Pages 169-184
  5. Following Synapse Dynamics

  6. Revealing Patterns of Synaptic Connectivity

    1. Front Matter
      Pages 291-291
    2. Mrinalini Hoon, Raunak Sinha, Haruhisa Okawa
      Pages 293-320
    3. Matthias G. Haberl, Melanie Ginger, Andreas Frick
      Pages 321-340
    4. Johan Winnubst, Christian Lohmann
      Pages 341-352
  7. Back Matter
    Pages 367-372

About this book


This detailed volume collects protocols for experimentation into how neurons connect to produce the extraordinary functionalities of the nervous system. Contributed by experts and pioneers in their respective techniques, the book covers synapses in the brain and in culture, their constituents, their structures, their dynamics, and the assemblies they form, all in the structure of a laboratory guide. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and  avoiding known pitfalls. 

Authoritative and practical, Synapse Development: Methods and Protocols serves as an ideal guide to minimizing the barrier to entry for the integration of new approaches with existing expertise, producing syntheses that will foster novel perspectives on the many ways in which synapses form, transform, and transmit.


Neurons Nervous system development Cell culture Synapse dynamics Synaptic constituents

Editors and affiliations

  • Alexandros Poulopoulos
    • 1
  1. 1.Department of Stem Cell and Regenerative BiologyHarvard UniversityCambridgeUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6686-8
  • Online ISBN 978-1-4939-6688-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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