Cardiac Gene Therapy

Methods and Protocols

  • Kiyotake Ishikawa

Part of the Methods in Molecular Biology book series (MIMB, volume 1521)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Overview

    1. Front Matter
      Pages 1-1
    2. Kiyotake Ishikawa, Roger J. Hajjar
      Pages 3-14
  3. Genetic Modification

    1. Front Matter
      Pages 15-15
    2. Henry Fechner, Roland Vetter, Jens Kurreck, Wolfgang Poller
      Pages 17-39
    3. Jimeen Yoo, Roger J. Hajjar, Dongtak Jeong
      Pages 41-53
    4. Vittavat Termglinchan, Timon Seeger, Caressa Chen, Joseph C. Wu, Ioannis Karakikes
      Pages 55-68
  4. Gene Delivery Vectors

    1. Front Matter
      Pages 89-89
    2. Andreas Jungmann, Oliver Müller, Kleopatra Rapti
      Pages 109-126
    3. Jason Kondrat, Nishat Sultana, Lior Zangi
      Pages 127-138
    4. Prabhu Mathiyalagan, Susmita Sahoo
      Pages 139-152
    5. Irene C. Turnbull, Ahmed A. Eltoukhy, Daniel G. Anderson, Kevin D. Costa
      Pages 153-166
  5. Gene Delivery Methods

    1. Front Matter
      Pages 167-167
    2. Kjetil Hodne, David B. Lipsett, William E. Louch
      Pages 169-182
    3. Francesca Stillitano, Ioannis Karakikes, Roger J. Hajjar
      Pages 183-193
    4. Federica del Monte, Kiyotake Ishikawa, Roger J. Hajjar
      Pages 195-204
    5. Shuyuan Chen, Paul A. Grayburn
      Pages 205-218
    6. Anthony S. Fargnoli, Michael G. Katz, Charles R. Bridges
      Pages 219-226
    7. Shin Watanabe, Lauren Leonardson, Roger J. Hajjar, Kiyotake Ishikawa
      Pages 227-235
    8. Guillaume Bonnet, Kiyotake Ishikawa, Roger J. Hajjar, Yoshiaki Kawase
      Pages 237-248
    9. Melissa J. Byrne, David M. Kaye
      Pages 261-269
    10. Michael G. Katz, Anthony S. Fargnoli, Andrew P. Kendle, Charles R. Bridges
      Pages 271-289
  6. Targeting Arrhythmia

    1. Front Matter
      Pages 291-291
    2. Patrick K. W. Chan, Ronald A. Li
      Pages 293-306
  7. Targeting Pulmonary Hypertension

    1. Front Matter
      Pages 323-323
    2. Lin Deng, Andrew H. Baker, Angela C. Bradshaw
      Pages 325-338
    3. Jaume Aguero, Lahouaria Hadri, Nadjib Hammoudi, Lauren Leonardson, Roger J. Hajjar, Kiyotake Ishikawa
      Pages 339-349
  8. Back Matter
    Pages 351-355

About this book


This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients’ outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Cardiac Gene Therapy: Methods and Protocols serves as a valuable tool for molecular biologists and physiologists in the cardiology field conducting cardiac gene transfer research, which will ultimately lead to further advancements in the vital field.


Vector technologies Cardiovascular disease treatment Cardiac gene transfer Vector delivery methods Gene transduction Cardiology Disease targets

Editors and affiliations

  • Kiyotake Ishikawa
    • 1
  1. 1.Cardiovascular Research CenterIcahn School of Medicine at Mount SinaiNew YorkUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6586-1
  • Online ISBN 978-1-4939-6588-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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