Drug Target miRNA

Methods and Protocols

  • Marco F. Schmidt

Part of the Methods in Molecular Biology book series (MIMB, volume 1517)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Reviews

    1. Front Matter
      Pages 1-1
    2. Marco F. Schmidt
      Pages 3-22
  3. Protocols

    1. Front Matter
      Pages 23-23
    2. Ioannis S. Vlachos, Artemis G. Hatzigeorgiou
      Pages 25-50
    3. Kim A. Lennox, Christopher A. Vakulskas, Mark A. Behlke
      Pages 51-69
    4. Geneviève Pépin, Jonathan Ferrand, Michael P. Gantier
      Pages 127-135
    5. Christopher L. Haga, Sai Pradeep Velagapudi, Jessica L. Childs-Disney, Jacqueline Strivelli, Matthew D. Disney, Donald G. Phinney
      Pages 179-198
    6. Johannes Danner, Balagopal Pai, Ludwig Wankerl, Gunter Meister
      Pages 199-210
    7. Teresa Bellissimo, Silvia Masciarelli, Elena Poser, Ilaria Genovese, Alberto Del Rio, Gianni Colotti et al.
      Pages 211-221
    8. Elena Poser, Ilaria Genovese, Silvia Masciarelli, Teresa Bellissimo, Francesco Fazi, Gianni Colotti
      Pages 223-237
    9. Marco F. Schmidt, Oliver Korb, Chris Abell
      Pages 239-249

About this book

Introduction

This volume provides a concise and technical discussion of recently developed approaches to overcome challenges, such as pharmacodynamics and pharmacokinetics difficulties, in miRNA drug discovery. These strategies cover anti-sense agents targeting miRNA that are applied in advanced formulations or are chemically optimized to increase delivery; small molecule miRNA modulators to overcome anti-sense agents’ limitations; general enhancers of miRNA maturation; and Argonaute 2 protein and its pharmacokinetic parameters. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Drug Target miRNA: Methods and Protocols is a valuable resource for anyone interested in the ever-evolving field of miRNA drug discovery.

 

Keywords

drug discovery pharmacodynamics pharmacokinetics small molecule miRNA modulators anti-sense agents DICER-mediated miRNA maturation Argonaute 2 protein oligonucleotides

Editors and affiliations

  • Marco F. Schmidt
    • 1
  1. 1.BEROCEUTICA GmbHPotsdamGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6563-2
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6561-8
  • Online ISBN 978-1-4939-6563-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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