T-Cell Differentiation

Methods and Protocols

  • Enrico Lugli

Part of the Methods in Molecular Biology book series (MIMB, volume 1514)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Camilla Jandus, Amaia Martínez Usatorre, Selena Viganò, Lianjun Zhang, Pedro Romero
    Pages 1-17
  3. Nicola Gagliani, Samuel Huber
    Pages 19-30
  4. Enrico Lugli, Veronica Zanon, Domenico Mavilio, Alessandra Roberto
    Pages 31-47
  5. Alexandre Pierre Benechet, Lucia Ganzer, Matteo Iannacone
    Pages 49-61
  6. Paola Larghi, Antonella Viola, Barbara Molon
    Pages 63-81
  7. Kristin L. Boswell, Takuya Yamamoto
    Pages 83-92
  8. Daisy Philips, Marlous van den Braber, Ton N. Schumacher, Pia Kvistborg
    Pages 93-101
  9. Francesco Annunziato, Laura Maggi
    Pages 127-137
  10. Eleonora Timperi, Vincenzo Barnaba, Silvia Piconese
    Pages 139-151
  11. Silvia Monticelli, Tarmo Äijö, Sara Trifari
    Pages 153-172
  12. Valeria Ranzani, Alberto Arrigoni, Grazisa Rossetti, Ilaria Panzeri, Sergio Abrignani, Raoul J. P. Bonnal et al.
    Pages 173-185
  13. Maria Dominguez, Mario Roederer, Pratip K. Chattopadhyay
    Pages 187-202
  14. Alexandra-Chloé Villani, Karthik Shekhar
    Pages 203-239
  15. Julie C. Ribot, Karine Serre, Bruno Silva-Santos
    Pages 257-267
  16. Back Matter
    Pages 269-271

About this book


This volume provides protocols to successfully apply cutting-edge technologies to characterize the biology of T cells at an unprecedented level of complexity. Chapters guide readers through flow cytometry and fluorescence-activated cell sorting, the behaviour of single T cells after adoptive cell transfer (ACT), single cell gene expression by multiplex PCR, lentiviral transduction approaches, protocols to derive large numbers of early-differentiated memory T cells by using dedicated cytokines cocktails, approaches to measure telomerase activity in terminally differentiated T cells, and approaches to define Treg cells at the phenotypic and functional level. The final part of the book is dedicated to the analysis of the differentiation and effector functions of innate T cells, namely the well-known γ/δ T cells, and the recently identified CD8+ mucosal associated invariant T (MAIT) cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, T-Cell Differentiation: Methods and Protocols aims to provide protocols that are fundamental to monitor the T cell compartment at the level of single cells in pathological and immunotherapy conditions.


memory T cell compartment fluorescence-activated cell sorting adoptive cell transfer intracellular proteins multiplex PCR

Editors and affiliations

  • Enrico Lugli
    • 1
  1. 1.Laboratory of Translational ImmunologyHumanitas Clinical & Research CenterRozzanoItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6546-5
  • Online ISBN 978-1-4939-6548-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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