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HDAC/HAT Function Assessment and Inhibitor Development

Methods and Protocols

  • Book
  • © 2017

Overview

Editors:
  1. Oliver H. Krämer

    1. Institute of Toxicology, University Medical Center Mainz, Mainz, Germany

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  • Includes cutting-edge methods and protocols to study HDACs and HATs
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1510)

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Table of contents (31 protocols)

  1. Assessment of HDAC-Dependent Effects on Signaling Cascades in Primary and Permanent Cells

    1. Phospho-Flow Analysis of Primary Mouse Cells After HDAC Inhibitor Treatment

      • Dagmar Hildebrand, Katharina F. Kubatzky
      Pages 233-243

    2. Evaluation of Innate Immune Gene Expression Following HDAC Inhibitor Treatment by High Throughput qPCR and PhosFlow Cytometry

      • David Olagnier, Cindy Chiang, John Hiscott
      Pages 245-255

    3. Assessing HDAC Function in the Regulation of Signal Transducer and Activator of Transcription 5 (STAT5) Activity Using Chromatin Immunoprecipitation (ChIP)

      • Sophia Pinz, Anne Rascle
      Pages 257-276

    4. Modulation of STAT1-Driven Transcriptional Activity by Histone Deacetylases

      • Benjamin Y. Owusu, Lidija Klampfer
      Pages 277-285

    5. Proximity Ligation Assay to Quantify Foxp3 Acetylation in Regulatory T Cells

      • Jing Jiao, Rongxiang Han, Wayne W. Hancock, Ulf H. Beier
      Pages 287-293

  2. PTMs Controlling Acetylation Balances and HDAC Functions

    1. Front Matter

      Pages 295-295
      Download chapter PDF

    2. Testing the Effects of SIAH Ubiquitin E3 Ligases on Lysine Acetyl Transferases

      • Jan Hagenbucher, Hilda Stekman, Alfonso Rodriguez-Gil, Michael Kracht, M. Lienhard Schmitz
      Pages 297-312

    3. Assessment of HDACi-Induced Acetylation of Nonhistone Proteins by Mass Spectrometry

      • Martin Wieczorek, Karl-Heinz Gührs, Thorsten Heinzel
      Pages 313-327

    4. Assessing the Role of Paralog-Specific Sumoylation of HDAC1

      • Simona Citro, Susanna Chiocca
      Pages 329-337

    5. Analysis of Histone Deacetylases Sumoylation by Immunoprecipitation Techniques

      • Tobias Wagner, Maren Godmann, Thorsten Heinzel
      Pages 339-351

  3. Improved, Novel, and Specific HDACi

    1. Front Matter

      Pages 353-353
      Download chapter PDF

    2. How to Distinguish Between the Activity of HDAC1-3 and HDAC6 with Western Blot

      • Mandy Beyer, Nicole Kiweler, Siavosh Mahboobi, Oliver H. Krämer
      Pages 355-364

    3. Evaluating the Effect of HDAC8 Inhibition in Malignant Peripheral Nerve Sheath Tumors

      • Gonzalo Lopez, Raphael E. Pollock
      Pages 365-374

    4. Analyzing the Impact of Pan- and Class-Specific HDACi on Differentiation-Associated Factors

      • Katrin Noack, Oliver H. Krämer
      Pages 375-385

    5. Encapsulation of the HDACi Ex527 into Liposomes and Polymer-Based Particles

      • Dorle Hennig, Diana Imhof
      Pages 387-398

    6. Preparation of Histone Deacetylase Inhibitor Vorinostat-Loaded Poly d, l-Lactide-co-Glycolide Polymeric Nanoparticles by Nanoprecipitation Method

      • Vilwanathan Ravikumar, Renu Sankar
      Pages 399-403

    7. Generation and Assessment of Fusions Between HDACi and TKi

      • Siavosh Mahboobi, Bernadette Pilsl, Andreas Sellmer
      Pages 405-412

  4. Back Matter

    Pages 413-415
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Keywords

About this book

This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be used to test experimental hypotheses. Written for the popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols serves as an ideal guide to this vital area of study.

Editors and Affiliations

  • Institute of Toxicology, University Medical Center Mainz, Mainz, Germany

    Oliver H. Krämer

Bibliographic Information

  • Book Title: HDAC/HAT Function Assessment and Inhibitor Development

  • Book Subtitle: Methods and Protocols

  • Editors: Oliver H. Krämer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-6527-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2017

  • Hardcover ISBN: 978-1-4939-6525-0Published: 20 October 2016

  • Softcover ISBN: 978-1-4939-8226-4Published: 20 June 2018

  • eBook ISBN: 978-1-4939-6527-4Published: 19 October 2016

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 415

  • Number of Illustrations: 39 b/w illustrations, 58 illustrations in colour

  • Topics: Protein Science

  • Industry Sectors: Biotechnology, Pharma

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