The Mitotic Exit Network

Methods and Protocols

  • Fernando Monje-Casas
  • Ethel Queralt

Part of the Methods in Molecular Biology book series (MIMB, volume 1505)

Table of contents

  1. Front Matter
    Pages i-x
  2. Analysis of Cell Cycle Progression and Mitotic Exit

  3. Biochemistry of the MEN

    1. Front Matter
      Pages 69-69
    2. Marco Geymonat, Adonis Spanos, Katrin Rittinger
      Pages 71-80
    3. Ethel Queralt, José-Antonio Rodriguez-Rodriguez
      Pages 89-96
    4. Neus Colomina, Clàudia Guasch, Jordi Torres-Rosell
      Pages 97-117
    5. Brendan L. Powers, Hana Hall, Harry Charbonneau, Mark C. Hall
      Pages 119-132
  4. Analysis of the Localization of MEN Components

  5. Control of Cellular Processes by the MEN

    1. Front Matter
      Pages 215-215
    2. Facundo Ramos, Joanne Leonard, Andrés Clemente-Blanco, Luis Aragón
      Pages 229-243
    3. Magdalena Foltman, Alberto Sanchez-Diaz
      Pages 245-262
  6. Final Remarks: The MEN as a Model for Higher Eukaryotes

    1. Front Matter
      Pages 263-263
  7. Back Matter
    Pages 279-281

About this book


This detailed book collects the main methodologies used for the analysis of the activity, localization, and regulation of the components of the Mitotic Exit Network (MEN) pathway during mitotic exit in Saccharomyces cerevisiae, as well as for the evaluation of the roles of these proteins in other cellular processes, such as the condensation of the rDNA, the functionality of the mitotic checkpoints, and cytokinesis. Budding yeast serves as an ideal model system for dissecting the mechanisms that regulate cell cycle progression and providing new insights into the molecular basis of cell cycle control and, thus, into the origin of diseases that arise as a consequence of problems during cell division. Therefore, although this volume concentrates on Saccharomyces cerevisiae as a model, it also details the implications that the research about the MEN have on our understanding of the mitotic exit process in higher eukaryotes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, The Mitotic Exit Network: Methods and Protocols will be a valuable reference for cellular and molecular biologists and biochemists as well as for all scientists interested in the study of the regulation of mitotic exit using budding yeast as a model organism.


Yeast models MEN pathway Saccharomyces cerevisiae Cell cycle progression MEN component localization Cell division

Editors and affiliations

  • Fernando Monje-Casas
    • 1
  • Ethel Queralt
    • 2
  1. 1.CABIMERSevillaSpain
  2. 2.Cancer Epigenetics & Biology ProgramIDIBELL Hospitalet de LlobregatBarcelonaSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6500-7
  • Online ISBN 978-1-4939-6502-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals