Mammary Gland Development

Methods and Protocols

  • Finian Martin
  • Torsten Stein
  • Jillian Howlin

Part of the Methods in Molecular Biology book series (MIMB, volume 1501)

Table of contents

  1. Front Matter
    Pages i-xi
  2. In Vivo Models

    1. Front Matter
      Pages 19-19
    2. Ayman M. Ibrahim, Claire Cairney, Joanna S. Morris, Torsten Stein
      Pages 149-164
    3. Bethan Lloyd-Lewis, Timothy J. Sargeant, Peter A. Kreuzaler, Henrike K. Resemann, Sara Pensa, Christine J. Watson
      Pages 165-186
  3. In Vitro 2D & 3D-Models

  4. Mammary Stem Cells

    1. Front Matter
      Pages 259-259
    2. Anoeska A. A. van de Moosdijk, Nai Yang Fu, Anne C. Rios, Jane E. Visvader, Renée van Amerongen
      Pages 291-308
  5. Translational Applications

    1. Front Matter
      Pages 309-309
    2. Laoighse Mulrane, William M. Gallagher, Darran P. O’Connor
      Pages 311-325
    3. Damir Varešlija, Sinead Cocchiglia, Christopher Byrne, Leonie Young
      Pages 327-336
  6. Back Matter
    Pages 337-358

About this book


This volume provides key molecular biological protocols and experimental strategies currently employed to study the various stages of mammary gland development. Chapters are divided into four parts covering genetically modified mouse models that exhibit prenatal and pubertal mammary gland phenotypes, 2D and 3D-model culture systems, methodologies for mammary stem cell isolation, and translational applications that provide a bridge between experimental studies of mammary gland development and the study of human breast cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mammary Gland Development: Methods and Protocols  will be useful for researchers whose primary interest is in mammary gland development; developmental biologists, epithelial cell biologists,  and those with an interest in molecular mechanisms underlying breast cancer.


genetically modified mouse models pubertal mammary gland phenotypes 2D and 3D-model culture systems stem cells mammary stem cell isolation human breast cancer

Editors and affiliations

  • Finian Martin
    • 1
  • Torsten Stein
    • 2
  • Jillian Howlin
    • 3
  1. 1.School of Biomolecular and Biomedical ScienceUniversity College DublinBelfieldIreland
  2. 2.Institute of Cancer Sciences College of MVLS University of GlasgowGlasgowUnited Kingdom
  3. 3.Division of Oncology-PathologyLund University Cancer Center/Medicon VillageLundSweden

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6473-4
  • Online ISBN 978-1-4939-6475-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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