In Vitro Mutagenesis

Methods and Protocols

  • Andrew Reeves

Part of the Methods in Molecular Biology book series (MIMB, volume 1498)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Gene and Genome-Editing Methods Part I

    1. Front Matter
      Pages 1-1
    2. Ciaran M. Lee, Haibao Zhu, Timothy H. Davis, Harshahardhan Deshmukh, Gang Bao
      Pages 3-21
    3. Kit-San Yuen, Chi-Ping Chan, Kin-Hang Kok, Dong-Yan Jin
      Pages 23-31
    4. Rongfang Xu, Pengcheng Wei, Jianbo Yang
      Pages 33-40
    5. Tetsushi Sakuma, Takuya Sakamoto, Takashi Yamamoto
      Pages 41-56
    6. Bang Shen, Kevin Brown, Shaojun Long, L. David Sibley
      Pages 79-103
  3. Gene and Genome-Editing Methods Part II

  4. Bioinformatics Approaches for Identifying and Analyzing Mutagenesis Targets

  5. In Vitro Transposon Mutagenesis Methods in Diverse Prokaryotic Systems

    1. Front Matter
      Pages 255-255
    2. J. Mark Weber, Andrew Reeves, William H. Cernota, Roy K. Wesley
      Pages 257-271
    3. Esteban Martínez-García, Tomás Aparicio, Víctor de Lorenzo, Pablo I. Nikel
      Pages 273-293
    4. Saija Kiljunen, Maria I. Pajunen, Harri Savilahti
      Pages 309-320
    5. Gaurav Majumdar, Rendani Mbau, Vinayak Singh, Digby F. Warner, Marte Singsås Dragset, Raju Mukherjee
      Pages 321-335
  6. Site-Directed Mutagenesis: PCR and DNA Polymerase-Based Methods

  7. In Vitro Mutagenesis for Studies of Protein Structure and Function

  8. Random Mutagenesis: Novel PCR-Based Methods

    1. Front Matter
      Pages 481-481
    2. Hong Feng, Hai-Yan Wang, Hong-Yan Zhao
      Pages 483-490
    3. Weilan Shao, Kesen Ma, Yilin Le, Hongcheng Wang, Chong Sha
      Pages 497-506
  9. Back Matter
    Pages 507-511

About this book


In vitro mutagenesis remains a critical experimental approach for investigating gene and protein function at the cellular level. This volume provides a wide variety of updated and novel approaches for performing in vitro mutagenesis using such methods as genome editing, transposon (Tn) mutagenesis, site-directed, and random mutagenesis. In Vitro Mutagenesis: Methods and Protocols guides readers through methods for gene and genome editing, practical bioinformatics approaches for identifying mutagenesis targets, and novel site-directed and random mutagenesis approaches aimed at gaining a better understanding of protein-protein and protein-cofactor interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, In Vitro Mutagenesis: Methods and Protocols aims to provide a highly accessible and practical manual for current and future molecular biology researchers, from the beginner practitioner to the advanced investigator in fields such as molecular genetics, biochemistry, and biochemical and metabolic engineering.


gene and genome editing facile genetic manipulation mutagenesis targets protein-cofactor interactions cellular metabolism mutagenic PCR methods

Editors and affiliations

  • Andrew Reeves
    • 1
  1. 1.Coskata, Inc.WarrenvilleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6470-3
  • Online ISBN 978-1-4939-6472-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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