Activity-Based Proteomics

Methods and Protocols

  • Herman S. Overkleeft
  • Bogdan I. Florea

Part of the Methods in Molecular Biology book series (MIMB, volume 1491)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Sander I. van Kasteren, Bogdan I. Florea, Herman S. Overkleeft
    Pages 1-8
  3. Explorative Activity-Based Proteomics

    1. Front Matter
      Pages 9-9
    2. Susanne Zweerink, Tanja Pollmann, Sabrina Ninck, Farnusch Kaschani, Markus Kaiser
      Pages 23-46
    3. Balakumaran Chandrasekar, Tram Ngoc Hong, Renier A. L. van der Hoorn
      Pages 47-56
    4. Joanna Krysiak, Stephan A. Sieber
      Pages 57-74
    5. Joanna Krysiak, Rolf Breinbauer
      Pages 87-99
  4. Medical Applications of Activity-Based Proteomics

    1. Front Matter
      Pages 101-101
    2. Laurette Prely, Theo Klein, Paul P. Geurink, Krisztina Paal, Herman S. Overkleeft, Rainer Bischoff
      Pages 103-111
    3. Benedikt M. Kessler, Sara Bursomanno, Joanna F. McGouran, Ian D. Hickson, Ying Liu
      Pages 131-144
    4. Laura E. Edgington-Mitchell, Matthew Bogyo, Martijn Verdoes
      Pages 145-159
    5. Marc P. Baggelaar, Mario Van der Stelt
      Pages 161-169
  5. Chemical Strategies in Activity-Based Proteomics

    1. Front Matter
      Pages 171-171
    2. Yinliang Yang, Marko Fonović, Steven H. L. Verhelst
      Pages 185-203
    3. Guillem Paniagua Soriano, Herman S. Overkleeft, Bogdan I. Florea
      Pages 205-215
  6. Back Matter
    Pages 217-222

About this book


This volume focuses on explorative activity-based proteomics, biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Activity-Based Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.


activity-based proteomics annotate enzyme families activity-based protein bioorthogonal chemistry analytical chemistry

Editors and affiliations

  • Herman S. Overkleeft
    • 1
  • Bogdan I. Florea
    • 2
  1. 1.Bio-Organic Synthesis Group Leiden Institute of ChemistryLeiden UniversityLeidenThe Netherlands
  2. 2.Bio-Organic Synthesis Group Leiden Institute of ChemistryLeiden UniversityLeidenThe Netherlands

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6437-6
  • Online ISBN 978-1-4939-6439-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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