Clostridium difficile

Methods and Protocols

  • Adam P. Roberts
  • Peter Mullany

Part of the Methods in Molecular Biology book series (MIMB, volume 1476)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Susan P. Sambol, Stuart Johnson, Dale N. Gerding
    Pages 1-13
  3. Muhammad Ehsaan, Sarah A. Kuehne, Nigel P. Minton
    Pages 35-52
  4. Eric M. Ransom, David S. Weiss, Craig D. Ellermeier
    Pages 53-67
  5. Carolina Piçarra Cassona, Fátima Pereira, Mónica Serrano, Adriano O. Henriques
    Pages 69-90
  6. Séverine Péchiné, Cécile Denève-Larrazet, Anne Collignon
    Pages 91-101
  7. Adrianne N. Edwards, Shonna M. McBride
    Pages 117-128
  8. Aimee Shen, Kelly A. Fimlaid, Keyan Pishdadian
    Pages 129-142
  9. Ognjen Sekulović, Louis-Charles Fortier
    Pages 143-165
  10. John P. Hegarty, William Sangster, Robert E. Ashley, Roland Myers, Susan Hafenstein, David B. Stewart Sr.
    Pages 167-175
  11. Shan Goh
    Pages 177-185
  12. Michael S. M. Brouwer, Peter Mullany, Elaine Allan, Adam P. Roberts
    Pages 215-222
  13. Grace S. Crowther, Mark H. Wilcox, Caroline H. Chilton
    Pages 223-234
  14. Jennifer M. Auchtung, Catherine D. Robinson, Kylie Farrell, Robert A. Britton
    Pages 235-258
  15. Elizabeth Perez, Christine H. Lee, Elaine O. Petrof
    Pages 259-267
  16. Richard R. Rustandi, Feng Wang, Catherine Lancaster, Adam Kristopeit, David S. Thiriot, Jon H. Heinrichs
    Pages 269-277
  17. Catherine Lancaster, Richard R. Rustandi, Paola Pannizzo, Sha Ha
    Pages 279-287
  18. Back Matter
    Pages 289-292

About this book


This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen Clostridium difficile. Chapters detail up-to -date experimental techniques for gene editing and transcriptional analysis which are used to investigate the fundamental biology of the organism and its virulence factors.  Additional chapters  describe development of potential new treatments including vaccines, bacteriophage and faecal transplantation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols, Second Edition provides a comprehensive catalogue of molecular tools and techniques authored by the researchers who have developed them.


molecular tools faecal transplantation vaccine development bacteriophage product therapy phage product therapy horizontal gene transfer variable genome

Editors and affiliations

  • Adam P. Roberts
    • 1
  • Peter Mullany
    • 2
  1. 1.Department of Microbial DiseasesUCL Eastman Dental Institute, University College LondonLondonUnited Kingdom
  2. 2.Department of Microbial DiseasesUCL Eastman Dental Institute, University College LondonLondonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6359-1
  • Online ISBN 978-1-4939-6361-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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