High-Resolution Imaging of Cellular Proteins

Methods and Protocols

  • Steven D. Schwartzbach
  • Omar Skalli
  • Thomas Schikorski

Part of the Methods in Molecular Biology book series (MIMB, volume 1474)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Molecular Toolbox

    1. Front Matter
      Pages 1-1
    2. Jay M. Bhatt, Melanie L. Styers, Elizabeth Sztul
      Pages 3-24
    3. Bao-Shiang Lee, Jin-Sheng Huang, Lasanthi P. Jayathilaka, Jenny Lee, Shalini Gupta
      Pages 25-47
    4. Masami Nakazawa, Mari Mukumoto, Kazutaka Miyatake
      Pages 49-59
    5. Mónica S. Montesinos, Rachel Satterfield, Samuel M. Young Jr.
      Pages 73-90
  3. Fluorescent Microscopy Toolbox

  4. Electron Microscopy Toolbox

    1. Front Matter
      Pages 201-201
    2. Harumi Harada, Ryuichi Shigemoto
      Pages 203-216
    3. Lou G. Boykins, Jonathan C. R. Jones, Carlos E. Estraño, Steven D. Schwartzbach, Omar Skalli
      Pages 217-232
    4. Miyuki Takeuchi, Keiji Takabe, Yoshinobu Mineyuki
      Pages 233-242
    5. Jindřiška Fišerová, Christine Richardson, Martin W. Goldberg
      Pages 243-258

About this book

Introduction

This volume presents authoritative and cutting-edge methods and protocols focusing on three tool boxes covering the increasingly diverse methodologies used to image selected proteins and to investigate their function by light and electron microscopy. The first tool box includes the development of a wide range of molecular and immunological probes to target specific proteins. The second details the use of these probes for high resolution fluorescence microscopy and the third focuses on applications for transmission and scanning electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls thus ensuring successful results in the further study of this vital field.

Keywords

molecular tool box epitope tagged proteins polyclonal antibodies anti-peptide antibodies fluorescent microscopy toolbox conventional fluorophores cryo-ultramicrotomy rapid freeze-replacement fixation

Editors and affiliations

  • Steven D. Schwartzbach
    • 1
  • Omar Skalli
    • 2
  • Thomas Schikorski
    • 3
  1. 1.Department of Biological SciencesThe University of MemphisMemphisUSA
  2. 2.Department of Biological SciencesThe University of MemphisMemphisUSA
  3. 3.Department of AnatomyUniversidad Central del CaribeBayamonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-6352-2
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6350-8
  • Online ISBN 978-1-4939-6352-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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