High-Throughput RNAi Screening

Methods and Protocols

  • David O. Azorsa
  • Shilpi Arora

Part of the Methods in Molecular Biology book series (MIMB, volume 1470)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Justin J. Montoya, David O. Azorsa
    Pages 15-24
  3. Carina von Schantz, Jani Saarela
    Pages 25-37
  4. Katarzyna Jastrzebski, Bastiaan Evers, Roderick L. Beijersbergen
    Pages 49-73
  5. Daniel F. Gilbert, Michael Boutros
    Pages 75-84
  6. Anupriya Agarwal, Jeffrey W. Tyner
    Pages 85-94
  7. Alessandro Ceroni, Geoff S. Higgins, Daniel V. Ebner
    Pages 103-119
  8. Linda P. O’Reilly, Ryan R. Knoerdel, Gary A. Silverman, Stephen C. Pak
    Pages 151-162
  9. Subhanita Ghosh, Gatikrushna Singh, Bindiya Sachdev, Ajit Kumar, Pawan Malhotra, Sunil K. Mukherjee et al.
    Pages 199-212
  10. Li-Chuan Tseng, Chih-Hao Tang, Yun-Jin Jiang
    Pages 213-224
  11. Tomohiro Miki, Yuki Nakaoka, Gohta Goshima
    Pages 225-246
  12. David O. Azorsa, Megan A. Turnidge, Shilpi Arora
    Pages 247-260
  13. Back Matter
    Pages 261-262

About this book


High-throughput RNAi screening remains one of the most widely used technologies to perform target identification and validation studies in an unbiased manner. These assays are equally important for research and development across academic, biotech, and pharmaceutical industries. The success of these screening efforts is dependent on robust methodologies to perform these screens. In High-Throughput RNAi Screening: Methods and Protocols, expert researchers in the field share protocols and methods for performing high-throughput RNAi (HT-RNAi) screens. These include the use of various RNAi platforms and delivery methods in mammalian and non-mammalian systems, whole organism and cell models, and various applications, such as drug sensitizer identification. Finally, the book examines the latest advancements in the fields of assay development, library screening, data analysis, and hit selection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, High-Throughput RNAi Screening: Methods and Protocols provides a comprehensive source of protocols and other necessary information to make robust and successful assays possible for all who wish to apply HT-RNAi in their research.




drug discovery short interfering RNA (siRNA) short hairpin RNA (shRNA) genomics HT-RNAi mammalian cells drug sensitizer identification C. elegans drosophila assay development library screening

Editors and affiliations

  • David O. Azorsa
    • 1
  • Shilpi Arora
    • 2
  1. 1.Institute of Molecular MedicinePhoenix Children’s HospitalPhoenixUSA
  2. 2.Constellation PharmaceuticalsCambridgeUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-6335-5
  • Online ISBN 978-1-4939-6337-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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