Chromosome and Genomic Engineering in Plants

Methods and Protocols

  • Minoru Murata

Part of the Methods in Molecular Biology book series (MIMB, volume 1469)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Nathaniel Graham, Nathan Swyers, Jon Cody, Morgan McCaw, Changzeng Zhao, James A. Birchler
    Pages 1-13
  3. Ruyu Li, Zhiguo Han, Lili Hou, Gurminder Kaur, Qian Yin, David W. Ow
    Pages 15-30
  4. Minoru Murata, Asaka Kanatani, Kazunari Kashihara
    Pages 63-76
  5. Hisashi Tsujimoto
    Pages 101-109
  6. Masaki Endo, Ayako Nishizawa-Yokoi, Seiichi Toki
    Pages 123-135
  7. Ayako Nishizawa-Yokoi, Hiroaki Saika, Seiichi Toki
    Pages 137-146
  8. Takehito Kobayashi, Yusuke Yagi, Takahiro Nakamura
    Pages 147-155
  9. Petr Cápal, Jan Vrána, Marie Kubaláková, Takashi R. Endo, Jaroslav Doležel
    Pages 157-170
  10. Nobuko Ohmido, Toshiyuki Wako, Seiji Kato, Kiichi Fukui
    Pages 171-180
  11. Fukashi Shibata
    Pages 181-188
  12. Satoru Fujimoto, Sachihiro Matsunaga, Minoru Murata
    Pages 207-216
  13. Back Matter
    Pages 217-218

About this book


This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a “plant chromosome vector” system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination. The second approach is “genome-editing,” which makes it possible to introduce mutations into any of the genes of DNA that we wish to change. In addition, this book also covers other related techniques used to accelerate progress in plant chromosome and genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Chromosome and Genomic Engineering in Plants: Methods and Protocols provides a comprehensive source of protocols and other necessary information to anyone interested in this field of study.



plant genetics & genomics DNA insertion plant chromosome vector plant artificial chromosomes Cre/LoxP Ac/DS system genome editing

Editors and affiliations

  • Minoru Murata
    • 1
  1. 1.Institute of Plant Science and ResourcesOkayama UniversityKurashikiJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-4929-8
  • Online ISBN 978-1-4939-4931-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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