The Wilms' Tumor (WT1) Gene

Methods and Protocols

  • Nicholas Hastie

Part of the Methods in Molecular Biology book series (MIMB, volume 1467)

Table of contents

  1. Front Matter
    Pages i-x
  2. Jocelyn Charlton, Kathy Pritchard-Jones
    Pages 1-14
  3. Eve Miller-Hodges
    Pages 15-21
  4. Bettina Wilm, Ramon Muñoz-Chapuli
    Pages 23-39
  5. Bettina Wilm, Ramon Muñoz-Chapuli
    Pages 41-59
  6. Víctor Velecela, Janat Fazal-Salom, Ofelia M. Martínez-Estrada
    Pages 61-71
  7. Sophie McHaffie, You-Ying Chau
    Pages 73-80
  8. Richard J. McGregor, R. Ogley, PWF Hadoke, Nicholas Hastie
    Pages 93-104
  9. Martin Lee, Alan Serrels
    Pages 105-118
  10. Birgit Perner, Thomas J. D. Bates, Uta Naumann, Christoph Englert
    Pages 119-128
  11. Thomas J. D. Bates, Uta Naumann, Christoph Englert
    Pages 133-136
  12. Stefan G. E. Roberts
    Pages 137-154
  13. Fabio da Silva, Filippo Massa, Andreas Schedl
    Pages 177-188
  14. Ruthrothaselvi Bharathavikru, Alex von Kriegsheim
    Pages 189-196
  15. Ruthrothaselvi Bharathavikru, Tatiana Dudnakova
    Pages 197-209
  16. Back Matter
    Pages 233-235

About this book


This volume provides a thorough overview of the Wilms’ Tumour Gene (WT1). The book begins with three review chapters that cover the involvement of WT1 in pediatric cancer, kidney disease, and tissue development and homeostasis. The next few chapters discuss cell marking and lineage tracing, epicardial cell methodology, colony forming assays for bone marrow stem cells, angiogenesis assays and zebrafish tools.  The next group of chapters explores the latest tools in genomics, molecular biology, and biochemistry. They discuss dissecting transcription factor function in cell free systems, ChiP seq, proteomics, RNA interactome, and multiphoton imaging of lipids, measuring the binding constants of protein-nucleic acid interactions, and bioinformatics approaches for analyzing Next Generation Sequence data. The final chapter discusses protocols for clinical trials for immune therapy using anti-WT1 peptides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, The Wilms’ Tumour (WT1) Gene: Methods and Protocols is a valuable resource for anyone who is interested in the diverse methodologies used in WT1 research.



multifunctional protein mesenchymal-epithelial switches zinc finger protein cell making lineage tracing zebrafish protein-nucleic acid interaction ChIP-Seq WT1-interacting RNA cancer immune therapy

Editors and affiliations

  • Nicholas Hastie
    • 1
  1. 1.MRC Human Genetics UnitUniversity of EdinburghEdinburghUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-4021-9
  • Online ISBN 978-1-4939-4023-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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