Germline Stem Cells

  • Michael Buszczak

Part of the Methods in Molecular Biology book series (MIMB, volume 1463)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Sarah L. Crittenden, Hannah S. Seidel, Judith Kimble
    Pages 1-33
  3. Julie N. R. Collins, James J. Collins III
    Pages 35-47
  4. Leah J. Greenspan, Erika L. Matunis
    Pages 63-74
  5. Dana Gancz, Lilach Gilboa
    Pages 75-83
  6. Nevine A. Shalaby, Michael Buszczak
    Pages 85-91
  7. Amanda M. Butler, Tristan Aguero, Karen M. Newman, Mary Lou King
    Pages 115-124
  8. Lei Lei, Allan C. Spradling
    Pages 125-138
  9. Gina M. Aloisio, Ileana Cuevas, Yuji Nakada, Christopher G. Peña, Diego H. Castrillon
    Pages 139-154
  10. Cassy M. Spiller, Guillaume Burnet, Josephine Bowles
    Pages 173-183
  11. Takamasa Hirano, Hidetoshi Hasuwa, Haruhiko Siomi
    Pages 205-216
  12. Back Matter
    Pages 227-229

About this book


This second edition provides updated and new chapters on selected genetic, molecular, biochemical, and cell biological techniques. Chapter’s guide readers through methods and principles on primordial germ cells and germline stem cells, however many of these principles can be applied to different types of adult stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Germline Stem Cells: Second Edition aims to present the new findings and techniques that have developed since the first edition.


Germline stem cell primordial germ cells adult stem cells live-cell imaging techniques improved cell purification germ cells

Editors and affiliations

  • Michael Buszczak
    • 1
  1. 1.Department of Molecular BiologyUniversity of Texas Southwestern Medical CenterDallasUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2017
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-4015-8
  • Online ISBN 978-1-4939-4017-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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