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Capillary Electrophoresis of Proteins and Peptides

Methods and Protocols

  • Nguyet Thuy Tran
  • Myriam Taverna

Part of the Methods in Molecular Biology book series (MIMB, volume 1466)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Isabelle Le Potier, Audrey Boutonnet, Vincent Ecochard, François Couderc
    Pages 1-10
  3. Elena Domínguez-Vega, Rob Haselberg, Govert W. Somsen
    Pages 25-41
  4. Fernando Benavente, Silvia Medina-Casanellas, Estela Giménez, Victoria Sanz-Nebot
    Pages 67-84
  5. Changlu Gao, Xiuhua Sun, Huaixin Wang, Wei Qiao, Bo Hu
    Pages 85-92
  6. Caitlyn A. G. De Jong, Jessica Risley, Alexis K. Lee, Shuai Sherry Zhao, David D. Y. Chen
    Pages 137-149
  7. Richard R. Rustandi, Melissa Hamm, Catherine Lancaster, John W. Loughney
    Pages 197-217
  8. Back Matter
    Pages 233-234

About this book

Introduction

This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. <
Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study.

Keywords

CE-MS Intact proteins Detection methods Therapeutic proteins Quality control Vaccine proteins Glycoprotein analysis

Editors and affiliations

  • Nguyet Thuy Tran
    • 1
  • Myriam Taverna
    • 2
  1. 1.Institut Galien Paris-Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRSUniv. Paris-Sud, Université Paris-SaclayChâtenay-MalabryFrance
  2. 2.Institut Galien Paris-Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRSUniv. Paris-Sud, Université Paris-SaclayChâtenay-MalabryFrance

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-4014-1
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-4012-7
  • Online ISBN 978-1-4939-4014-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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