The Nucleolus

Methods and Protocols

  • Attila Németh

Part of the Methods in Molecular Biology book series (MIMB, volume 1455)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Imaging of the Nucleolus

    1. Front Matter
      Pages 1-1
    2. Marjolein van Sluis, Chelly van Vuuren, Brian McStay
      Pages 3-14
    3. Christophe Normand, Maxime Berthaud, Olivier Gadal, Isabelle Léger-Silvestre
      Pages 29-40
    4. Robert M. Martin, Henry D. Herce, Anne K. Ludwig, M. Cristina Cardoso
      Pages 71-82
  3. Analysis of Ribosomal RNA Transcription and Processing

    1. Front Matter
      Pages 83-83
    2. Michael Pilsl, Philipp E. Merkl, Philipp Milkereit, Joachim Griesenbeck, Herbert Tschochner
      Pages 99-108
    3. Victor Y. Stefanovsky, Tom Moss
      Pages 133-145
    4. Minshi Wang, Dimitri G. Pestov
      Pages 147-157
  4. Genomics and Proteomics of the Nucleolus

    1. Front Matter
      Pages 159-159
    2. Frédéric Pontvianne, Myriam Boyer-Clavel, Julio Sáez-Vásquez
      Pages 203-211
    3. Astrid Bruckmann, Jan Linnemann, Jorge Perez-Fernandez
      Pages 213-223
    4. Dalila Bensaddek, Armel Nicolas, Angus I. Lamond
      Pages 249-262
    5. Armel Nicolas, Dalila Bensaddek, Angus I. Lamond
      Pages 263-276
  5. Back Matter
    Pages 277-279

About this book


This volume provides an up-to-date compilation of current methodological approaches utilized for the exploration of nucleolar structure and function. Chapters cover a diversity of protocols that include imaging of the nucleolus, analysis of ribosomal RNA transcription and processing, and genomics and proteomics of the nucleolus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, The Nucleolus: Methods and Protocols provides scientists with a reliable practical handbook to facilitate the investigation of this nuclear compartment at the advanced level.


ribosomal RNA nucleolus Genomics of the nucleolus proteomics of the nucleolus fluorescence in situ hybridization immunofluorescence

Editors and affiliations

  • Attila Németh
    • 1
  1. 1.Department of Biochemistry IIIUniversity of RegensburgRegensburgGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3790-5
  • Online ISBN 978-1-4939-3792-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals