Plant Proteostasis

Methods and Protocols

  • L. Maria Lois
  • Rune Matthiesen

Part of the Methods in Molecular Biology book series (MIMB, volume 1450)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Ubiquitin Conjugation and Deconjugation Analysis

    1. Front Matter
      Pages 1-1
    2. Qian Chen, Xiaoyuan Yang, Qi Xie
      Pages 3-10
    3. Antje Hellmuth, Luz Irina A. Calderón Villalobos
      Pages 23-34
    4. Kamila Kalinowska, Marie-Kristin Nagel, Erika Isono
      Pages 35-44
    5. Katarzyna Zientara-Rytter, Agnieszka Sirko
      Pages 45-54
    6. Christin Naumann, Augustin C. Mot, Nico Dissmeyer
      Pages 55-83
    7. Maria Klecker, Nico Dissmeyer
      Pages 85-94
  3. Ubiquitin-like Protein Conjugation, Deconjugation, and Cell Imaging Studies

    1. Front Matter
      Pages 95-95
    2. Konstantin Tomanov, Ionida Ziba, Andreas Bachmair
      Pages 97-105
    3. Laura Castaño-Miquel, L. Maria Lois
      Pages 107-123
    4. Gary Yates, Anjil Srivastava, Beatriz Orosa, Ari Sadanandom
      Pages 125-133
    5. María Esther Pérez-Pérez, Ascensión Andrés-Garrido, José L. Crespo
      Pages 151-159
    6. Yunting Pu, Diane C. Bassham
      Pages 161-172
  4. Proteomic Analysis and Other Post-Translational Modification Studies

    1. Front Matter
      Pages 173-173
    2. Séverine Planchais, Laurent Camborde, Isabelle Jupin
      Pages 175-194
    3. Marc Planas-Marquès, Saul Lema A., Núria S. Coll
      Pages 195-203
    4. Qiong Xia, Hayat El-Maarouf-Bouteau, Christophe Bailly, Patrice Meimoun
      Pages 205-212
    5. Isabel Cristina Vélez-Bermúdez, Tuan-Nan Wen, Ping Lan, Wolfgang Schmidt
      Pages 213-221
    6. Frederik Faden, Lennart Eschen-Lippold, Nico Dissmeyer
      Pages 247-258
  5. Bioinformatics Analysis

    1. Front Matter
      Pages 259-259
    2. Lorenzo Carretero-Paulet, Victor A. Albert
      Pages 261-272
    3. Pedro Humberto Castro, Miguel Ângelo Santos, Alexandre Papadopoulos Magalhães, Rui Manuel Tavares, Herlânder Azevedo
      Pages 285-301
  6. Back Matter
    Pages 303-307

About this book


This volume is a collection of detailed protocols describing state-of-art approaches that will facilitate the understanding of protein homeostasis in plant stress responses and development. Plant Proteostasis: Methods and Protocols is broken into four parts focusing on the study of ubiquitin-dependent post-translational modifications, protocols focused on Ubl post-translational modifications, protein homeostasis, and protocols for the in silico analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols aims to address next challenges in agriculture such as precision horticulture.


Intracellular pathogens Microscopy SUMO Ubitquitin expression arrays

Editors and affiliations

  • L. Maria Lois
    • 1
  • Rune Matthiesen
    • 2
  1. 1.Development Program, CRAG (CSIC-IRTA-UAB-UB) Edifici CRAGCampus, UAB Bellaterra (Cerdanyola del Vallés)BarcelonaSpain
  2. 2.Computational and Experimental Biology Group, National Health Institute Doctor Ricardo Jorge, IPLisbonPortugal

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3757-8
  • Online ISBN 978-1-4939-3759-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Consumer Packaged Goods