Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools

  • Maurizio Federico

Part of the Methods in Molecular Biology book series (MIMB, volume 1448)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Recent Improvements in LV Construction, Production, and Transduction

  3. New LV Targets and Applications

    1. Front Matter
      Pages 63-63
    2. Maike Stahlhut, Axel Schambach, Olga S. Kustikova
      Pages 65-76
    3. Anil Chandrashekran, Colin Casimir, Nick Dibb, Carol Readhead, Robert Winston
      Pages 95-106
    4. Wei Wang, Cynthia C. Bartholomae, Richard Gabriel, Annette Deichmann, Manfred Schmidt
      Pages 107-120
    5. Elisabeth Pfeiffenberger, Reinhard Sigl, Stephan Geley
      Pages 121-138
  4. Integrase-Mutant Lvs

    1. Front Matter
      Pages 157-157
    2. Fazlina Nordin, Zariyantey Abdul Hamid, Lucas Chan, Farzin Farzaneh, M. K. Azaham A. Hamid
      Pages 159-173
    3. Ngoc B. Lu-Nguyen, Martin Broadstock, Rafael J. Yáñez-Muñoz
      Pages 175-184
    4. Diana Schenkwein, Seppo Ylä-Herttuala
      Pages 185-198
  5. Production, Detection, and Engineering of Exosomes

    1. Front Matter
      Pages 199-199
    2. Florian Dreyer, Andreas Baur
      Pages 201-216
    3. Carolina Coscia, Isabella Parolini, Massimo Sanchez, Mauro Biffoni, Zaira Boussadia, Cristiana Zanetti et al.
      Pages 217-235
    4. Zulfiqar A. Malik, Tingting T. Liu, Anne A. Knowlton
      Pages 237-248
    5. Francesco Manfredi, Paola Di Bonito, Claudia Arenaccio, Simona Anticoli, Maurizio Federico
      Pages 249-260
    6. Shin-ichiro Ohno, Masahiko Kuroda
      Pages 261-270
  6. Back Matter
    Pages 271-276

About this book


This volume provides current methods and protocols for gene and protein delivery based on both lentivirus-generated and spontaneously released nanovesicles.  Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools guides readers through methods on macromolecule delivery and chapters describe the LV-based protocols of gene engineering. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Lentiviral Vectors and Exosomes as Gene and Protein Delivery Tools aims to ensure successful results in the further study of this vital field.


cell engineering LV-producer cells hematopoietic stem cells spermatozoa hepatocytes

Editors and affiliations

  • Maurizio Federico
    • 1
  1. 1.Istituto Superiore di SanitàNational AIDS CenterRomeItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3751-6
  • Online ISBN 978-1-4939-3753-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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