Protein Tyrosine Phosphatases

Methods and Protocols

  • Rafael Pulido

Part of the Methods in Molecular Biology book series (MIMB, volume 1447)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Andrés Alonso, Caroline E. Nunes-Xavier, Yolanda Bayón, Rafael Pulido
    Pages 1-23
  3. Caroline E. Nunes-Xavier, Rafael Pulido
    Pages 25-37
  4. Mihaela Mentel, Rodica A. Badea, Georgiana Necula - Petrareanu, Sujay T. Mallikarjuna, Aura E. Ionescu, Stefan E. Szedlacsek
    Pages 39-66
  5. Liesbeth Hovestad-Bijl, Jeroen van Ameijde, Dirk Pijnenburg, Riet Hilhorst, Rob Liskamp, Rob Ruijtenbeek
    Pages 67-78
  6. Sandra Luna, Janire Mingo, Olaia Aurtenetxe, Lorena Blanco, Laura Amo, Jan Schepens et al.
    Pages 79-93
  7. Carlos Romá-Mateo, Madushi Raththagala, Mathew S. Gentry, Pascual Sanz
    Pages 107-119
  8. Yunpeng Bai, Zhi-Hong Yu, Zhong-Yin Zhang
    Pages 121-138
  9. Shantá D. Hinton
    Pages 139-153
  10. Colin Levy, James Adams, Lydia Tabernero
    Pages 155-180
  11. Sina Koch, Irene Helbing, Sylvia-Annette Böhmer, Makoto Hayashi, Lena Claesson-Welsh, Ola Söderberg et al.
    Pages 217-242
  12. Eynat Finkelshtein, Einat Levy-Apter, Ari Elson
    Pages 283-300
  13. Souad Rahmouni, Alexandre Hego, Céline Delierneux, Odile Wéra, Lucia Musumeci, Lutz Tautz et al.
    Pages 301-330
  14. Mathieu Amand, Charlotte Erpicum, Christine Gilles, Agnès Noël, Souad Rahmouni
    Pages 331-349
  15. Alexander James Hale, Jeroen den Hertog
    Pages 351-372
  16. Almudena Sacristán-Reviriego, María Molina, Humberto Martín
    Pages 385-398
  17. Back Matter
    Pages 399-402

About this book


This book provides coverage, methodology, and laboratory protocols on the more essential aspects of protein tyrosine phosphatase (PTP) function and regulation, including the use of standardized in vitro functional assays, suitable cell systems, and animal and microorganism models. Chapters covering state-of-the-art technical approaches suitable to decipher the physiologic roles of PTPs, and their involvement in tissue-specific functions, are also included, which will be of utility for both newcomers and experienced researchers in the field of tyrosine- and phosphoinositide- phosphorylation/dephosphorylation. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. <
Authoritative and practical, Protein Tyrosine Phosphatases: Methods and Protocols aims to aid researchers in better defining the common and individual features of the PTP family members and translating this knowledge into PTP-based therapy for human disease.


Cell signaling proteins PTP function and regulation Phosphorylation Dephosphorylation Protein kinases PTP family members Therapy for human disease

Editors and affiliations

  • Rafael Pulido
    • 1
  1. 1.BioCruces Health Research InstituteBarakaldo, BizkaiaSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3744-8
  • Online ISBN 978-1-4939-3746-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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