Natural Killer Cells

Methods and Protocols

  • Srinivas S. Somanchi

Part of the Methods in Molecular Biology book series (MIMB, volume 1441)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Aimee M. Beaulieu, Joseph C. Sun
    Pages 1-12
  3. Alexander W. Kay, Dara M. Strauss-Albee, Catherine A. Blish
    Pages 13-26
  4. Molly P. Keppel, Megan A. Cooper
    Pages 27-42
  5. Amy K. Erbe, Wei Wang, Mikayla Gallenberger, Jacquelyn A. Hank, Paul M. Sondel
    Pages 43-56
  6. Karin Tomin, Ronald H. Goldfarb, Per Albertsson
    Pages 65-74
  7. Saravanan Nandagopal, Francis Lin, Sam K. P. Kung
    Pages 75-86
  8. Bruno Vanherberghen, Thomas Frisk, Elin Forslund, Per E. Olofsson, Karolin Guldevall, Björn Önfelt
    Pages 87-106
  9. Kelsey J. McCulley, Srinivas S. Somanchi
    Pages 107-116
  10. Minh-Trang Thi Phan, Seung-Hwan Lee, Sang-Ki Kim, Duck Cho
    Pages 167-174
  11. Natalia Lapteva, Robin Parihar, Lisa A. Rollins, Adrian P. Gee, Cliona M. Rooney
    Pages 195-202
  12. Joshua N. Kellner, Conrad R. Cruz, Catherine M. Bollard, Eric S. Yvon
    Pages 203-213
  13. Emily R. Levy, Mattias Carlsten, Richard W. Childs
    Pages 231-240

About this book

Introduction

This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study NK cell migration and cytotoxicity through endpoint analysis or live single cell imaging are also discussed. Chapters also describe methods pertaining to translational application of NK cells, such as ex vivo expansion of NK cells on K562 cell lines genetically modified to express either membrane bound IL-15 or membrane bound IL-21, large scale NK cell culture, current techniques for engineering NK cells to express chimeric antigen receptors or chemokine receptors using retroviral vectors, electroporation of mRNA, and the natural phenomenon of trogocytosis. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting edge and thorough, Natural Killer Cells: Methods and Protocols is a valuable resource for researchers who not only want to understand mechanisms that govern NK cell behavior and diversity, but also for those who want to understand how to systematically evaluate NK cells for adoptive immunotherapy applications.

 

Keywords

cytotoxicity genotyping single nucleotide polymorphisms chemeric antigen receptors retroviral vectors electroporation of mRNA siRNA knockdown of gene expression in natural killer Cells natural kill cell adoptive immunotherapies fluorescence MRI imaging lymphocytes

Editors and affiliations

  • Srinivas S. Somanchi
    • 1
  1. 1.Division of PediatricsM.D. Anderson Cancer CenterHoustonUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-3684-7
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3682-3
  • Online ISBN 978-1-4939-3684-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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